Alamolhoda Mahboobeh, Mokhtari-Dizaji Manijhe, Barati Amir Hoshang, Hasanzadeh Hadi
Department of Medical Physics, Tarbiat Modares University, P.O. Box: 14115-133, Tehran, Iran.
Medical Physics Department, Kurdistan Medical Sciences University, Sanandaj, Iran.
J Med Ultrason (2001). 2012 Jul;39(3):115-25. doi: 10.1007/s10396-012-0348-9. Epub 2012 Mar 20.
Dual-frequency ultrasound has recently been shown to extensively enhance the acoustic cavitation yield in water.
In this study, the in vivo antitumor effect of simultaneous, dual-frequency ultrasound at low-level intensity (I SATA = 2 W/cm(2) for 1 MHz and I SATA = 0.2 W/cm(2) for 150 kHz) in combination with an intravenous injection of 5 mg/kg hematoporphyrin (Hp) was investigated in a model of breast adenocarcinoma in Balb/c mice. Seventy-one tumor-bearing mice were divided into nine treatment groups: control, sham, Hp injection, and single- and dual-frequency sonication in the presence and absence of Hp. The tumor growth delay was then calculated based on the tumor volume at various times after treatment using the following parameters: relative volume percent, [Formula: see text] and [Formula: see text] times (to reach five and two times the initial volume), percent of tumor growth inhibition ratio, and survival period.
Our results showed no significant difference between the 150 kHz and 1 MHz single-frequency groups when compared with the sham group after 9 days of treatment (p > 0.05). However, treatment with dual-frequency ultrasound significantly delayed tumor growth when compared with the sham group (p < 0.05) after 9 days of treatment. Furthermore, in vivo experiments showed that combined dual-frequency sonication controlled tumor growth more effectively than single-frequency sonication. Evaluation of the therapeutic effects of single- and dual-frequency ultrasound in sonodynamic therapy revealed that treatment with the combination of dual-frequency ultrasound and Hp resulted in a significant reduction in the relative volume percent of tumors after 3 days of treatment (p < 0.05) compared with the controls. Additionally, the T 5 time and the survival period in the group treated with the combination therapy was significantly longer than those in all the other groups (p < 0.05). These findings were further verified histopathologically.
In conclusion, our results reveal that sonodynamic therapy using dual-frequency ultrasound is able to extend the survival time of animals compared with single-frequency sonication.
最近研究表明，双频超声可显著提高水中的声空化产率。
在本研究中，在Balb/c小鼠乳腺癌模型中，研究了低强度（1MHz时I SATA = 2W/cm²，150kHz时I SATA = 0.2W/cm²）的同步双频超声联合静脉注射5mg/kg血卟啉（Hp）的体内抗肿瘤效果。71只荷瘤小鼠被分为9个治疗组：对照组、假处理组、Hp注射组以及在有和没有Hp的情况下进行单频和双频超声处理组。然后根据治疗后不同时间的肿瘤体积，使用以下参数计算肿瘤生长延迟：相对体积百分比、[公式：见原文]和[公式：见原文]倍（达到初始体积的5倍和2倍）、肿瘤生长抑制率百分比和生存期。
我们的结果显示，治疗9天后，150kHz和1MHz单频组与假处理组相比无显著差异（p > 0.05）。然而，治疗9天后，与假处理组相比，双频超声治疗显著延迟了肿瘤生长（p < 0.05）。此外，体内实验表明，双频超声联合处理比单频超声更有效地控制肿瘤生长。单频和双频超声在声动力治疗中的治疗效果评估显示，与对照组相比，双频超声与Hp联合治疗3天后肿瘤相对体积百分比显著降低（p < 0.05）。此外，联合治疗组的T 5时间和生存期显著长于所有其他组（p < 0.05）。这些发现通过组织病理学进一步得到验证。
总之,我们的结果表明，与单频超声相比，双频超声声动力治疗能够延长动物的生存时间。
