Hasanzadeh Hadi, Mokhtari-Dizaji Manijhe, Bathaie S Zahra, Hassan Zuhair M
Department of Medical Physics, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
Department of Medical Physics, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran.
J Med Ultrason (2001). 2013 Oct;40(4):303-8. doi: 10.1007/s10396-013-0438-3. Epub 2013 Mar 30.
The goal of this study was to localize drug release from nanomicelles using dual-frequency sonication at low levels of acoustic intensity.
In this study, the antitumor effect of simultaneous dual-frequency sonication (28 kHz and 3 MHz) at low levels of acoustic intensity in combination with doxorubicin and micellar doxorubicin injection was assessed in a spontaneous model of breast adenocarcinoma in female Balb/c mice. Sixty-three tumor-bearing mice were randomly grouped into control, sham, dual-frequency sonication, doxorubicin injection with and without dual-frequency sonication, and micellar doxorubicin injection with and without dual-frequency sonication groups.
The results of volume change relative to initial volume showed that in the micellar doxorubicin injection with sonication group, this parameter was significantly different from that of the control, sham, sonication, and doxorubicin injection groups (P < 0.05). In addition, the volume began to increase on the 15th day after the start of treatment, which is a good indication to repeat treatment; therefore, another group received an extra treatment on day 15. The animal life span in the micellar doxorubicin with sonication and repeated treatment groups was significantly higher than that in all the other experimental groups except for the micellar doxorubicin injection group (P < 0.05).
It was concluded that dual-frequency sonication with micellar doxorubicin injection extends the life span relative to doxorubicin injection or dual-frequency sonication alone, and that repeating this treatment on day 15 decreases the rate of tumor growth significantly.
本研究的目的是利用低强度声强的双频超声来定位纳米胶束中的药物释放。
在本研究中，在雌性Balb/c小鼠的自发性乳腺腺癌模型中，评估了低强度声强下同时进行双频超声（28kHz和3MHz）联合阿霉素和胶束阿霉素注射的抗肿瘤效果。将63只荷瘤小鼠随机分为对照组、假手术组、双频超声组、阿霉素注射组（有无双频超声）和胶束阿霉素注射组（有无双频超声）。
相对于初始体积的体积变化结果显示，在胶束阿霉素注射加超声组中，该参数与对照组、假手术组、超声组和阿霉素注射组有显著差异（P<0.05）。此外，治疗开始后第15天体积开始增加，这是重复治疗的良好指征；因此，另一组在第15天接受了额外治疗。胶束阿霉素加超声及重复治疗组的动物寿命明显高于除胶束阿霉素注射组外的所有其他实验组（P<0.05）。
得出的结论是，胶束阿霉素注射联合双频超声相对于单独的阿霉素注射或双频超声可延长寿命，并且在第15天重复这种治疗可显著降低肿瘤生长速率。
