Urbanics R, Greenberg J H, Toffano G, Reivich M
Department of Neurology, University of Pennsylvania, Philadelphia 19104-6063.
Stroke. 1989 Jun;20(6):795-802. doi: 10.1161/01.str.20.6.795.
The effect of the ganglioside GM1 was studied in a focal cerebral ischemia model in 30 cats consisting of 2 hours of middle cerebral artery occlusion followed by 4 hours of recirculation. The cerebrocortical electrical activity, extracellular potassium activity, and microcirculation indicated by NAD/NADH fluorescence were measured during occlusion as well as during recirculation in the core of the middle cerebral artery territory, while the cerebral metabolic rate for glucose (ICMRgl) was measured at the end of recirculation. The cats were classified into either mildly or moderately severe stroke groups based on the depression of the cerebrocortical electrical activity on the occluded side. Of 12 cats with only a mild stroke, six were administered GM1 intravenously 30 minutes after occlusion, while six cats were not treated. Of 12 cats with a moderate stroke, six were treated and six were left untreated. In six additional cats, only a sham insult was undertaken. In the cats with mild stroke, GM1 treatment significantly increased lCMRgl in the peripheral middle cerebral artery territory compared with the untreated cats; for the six treated cats, lCMRgl was normalized toward the control level, whereas it was depressed in the six untreated cats. There were no other significant effects of GM1 treatment on the other measured parameters. A potential protective effect of anesthesia is discussed.
在30只猫的局灶性脑缺血模型中研究了神经节苷脂GM1的作用，该模型包括大脑中动脉闭塞2小时，随后再灌注4小时。在大脑中动脉区域核心的闭塞期间以及再灌注期间，测量脑皮质电活动、细胞外钾活性以及由NAD/NADH荧光指示的微循环，同时在再灌注结束时测量脑葡萄糖代谢率（ICMRgl）。根据闭塞侧脑皮质电活动的抑制情况，将猫分为轻度或中度严重中风组。在12只仅有轻度中风的猫中，6只在闭塞后30分钟静脉注射GM1，而6只猫未接受治疗。在12只中度中风的猫中，6只接受治疗，6只未接受治疗。另外6只猫仅进行假手术。在轻度中风的猫中，与未治疗的猫相比，GM1治疗显著增加了大脑中动脉外周区域的ICMRgl；对于6只接受治疗的猫，ICMRgl恢复到对照水平，而6只未治疗的猫则降低。GM1治疗对其他测量参数没有其他显著影响。讨论了麻醉的潜在保护作用。
