Tanaka K, Dora E, Greenberg J H, Reivich M
Stroke. 1986 Sep-Oct;17(5):994-1004. doi: 10.1161/01.str.17.5.994.
Local cerebral glucose utilization (lCMRgl), NADH fluorescence, cerebral blood flow (CBF), electrocortical activity (ECoG) and histology were studied during a 4 hr recovery period following 2 hrs of left middle cerebral artery (MCA) occlusion in cats. Changes in relative reduced pyridine nucleotides and CBF were measured by fluororeflectometry, ECoG was obtained from the left middle ectosylvian gyrus (MEG), and lCMRgl was measured at the end of the recovery period autoradiographically with 14-C-2-deoxyglucose. A sham group was comprised of 4 cats. The ten animals subjected to the stroke were classified into 3 groups based on the mean amplitude of the ECoG at the end of the ischemic period. At the end of the recovery period, the relative reduced pyridine nucleotides showed a 22.5% oxidation (oxidation of NADH), a 66.2% reduction (reduction of NAD) and a 3.0% reduction compared to the sham group in the severe, moderate and mild groups, respectively. LCMRgl of the left MEG in the severe group was 64.2% of the corresponding sham value, whereas lCMRgl in the moderate and mild groups were 124.8% and 132.0% of the sham, respectively. CBF at the end of the recovery period ranged from 28.1% to 83.0% of the sham value, although there was no significant difference among these groups. Histologically, a large portion of the neurons in the left MEG in the severe group showed ischemic neuronal changes, while the damage was less severe in the moderate and mild groups. On the basis of these data, it is suggested that a relative substrate deficiency and/or a loss of mitochondrial enzymatic pool size may occur in the animals comprizing the severe group. Conversely, anaerobic glycolysis may be activated in the moderate group, while the mild group exhibits an increase in glucose metabolism that is most likely aerobic. A gradient in the magnitude of changes in lCMRgl was noted from the central MCA territory to the surrounding brain regions in the ischemic hemisphere. In addition, there was a mild, but statistically significant (p less than 0.05), depression in lCMRgl with no histological damage in the non-ischemic hemisphere of the severe group.
在猫左侧大脑中动脉（MCA）闭塞2小时后的4小时恢复期间，对局部脑葡萄糖利用率（lCMRgl）、NADH荧光、脑血流量（CBF）、皮层电活动（ECoG）和组织学进行了研究。通过荧光反射法测量相对还原吡啶核苷酸和CBF的变化，从左侧外侧薛氏回（MEG）获取ECoG，并在恢复期末用14-C-2-脱氧葡萄糖放射自显影法测量lCMRgl。假手术组由4只猫组成。根据缺血期末ECoG的平均振幅，将10只中风动物分为3组。在恢复期末，与假手术组相比，重度、中度和轻度组的相对还原吡啶核苷酸分别显示22.5%的氧化（NADH氧化）、66.2%的还原（NAD还原）和3.0%的降低。重度组左侧MEG的lCMRgl为相应假手术值的64.2%，而中度和轻度组的lCMRgl分别为假手术值的124.8%和132.0%。恢复期末的CBF为假手术值的28.1%至83.0%，尽管这些组之间无显著差异。组织学上，重度组左侧MEG的大部分神经元显示缺血性神经元变化，而中度和轻度组的损伤较轻。基于这些数据，提示重度组动物可能发生相对底物缺乏和/或线粒体酶库大小的丧失。相反，中度组可能激活无氧糖酵解，而轻度组表现出最可能为有氧的葡萄糖代谢增加。在缺血半球，从大脑中动脉中央区域到周围脑区，lCMRgl的变化幅度存在梯度。此外，重度组非缺血半球的lCMRgl有轻度但具有统计学意义（p小于0.05）的降低，且无组织学损伤。
