Kawai H, Isobe Y, Horibe M, Tokuda J, Tokuno I, Endo K, Kawai F
a Department of Food Science and Nutrition , Nara Women's University , Nara 630 , Japan.
b Department of Biology , Kobe University of Commerce , Kobe 655 , Japan.
Biosci Biotechnol Biochem. 1992 Jan;56(6):853-7. doi: 10.1271/bbb.56.853.
A bacterium that was isolated from soil and identified as Bacillus circulans was found to produce a highly viscous extracellular polysaccharide when it was grown aerobically in a medium containing glucose as a sole source of carbon. The product was characterized by TLC and GC analyses as a novel heteropolysaccharide consisted of rhamnose, mannose, galactose, and mannuronic acid as sugar components. A maximal yield of polysaccharide reached about 2 g/liter by jar-fermentor culture at 30°C for 48 hr with a medium containing 1% glucose, 0.05% asparagine, 0.005% yeast extract, and small amounts of inorganic salts. Some culture conditions for the production of polysaccharide were investigated with flask culture; an optimal production was attained with a medium containing 0.1-1 % glucose and 0.01-0.05% asparagine, pH 7-8, at 30°C under aerobic conditions.
从土壤中分离出的一种被鉴定为环状芽孢杆菌的细菌，当它在以葡萄糖作为唯一碳源的培养基中好氧生长时，被发现能产生一种高粘性的细胞外多糖。通过薄层色谱（TLC）和气相色谱（GC）分析，该产物被表征为一种新型杂多糖，其糖成分包括鼠李糖、甘露糖、半乳糖和甘露糖醛酸。在含有1%葡萄糖、0.05%天冬酰胺、0.005%酵母提取物和少量无机盐的培养基中，于30°C用罐式发酵罐培养48小时，多糖的最大产量达到约2克/升。用摇瓶培养研究了多糖生产的一些培养条件；在有氧条件下，于30°C，使用含有0.1 - 1%葡萄糖和0.01 - 0.05%天冬酰胺、pH值为7 - 8的培养基可实现最佳生产。
