Cooper J K, Hawes E M, Hubbard J W, McKay G, Midha K K
College of Pharmacy, University of Saskatchewan, Saskatoon, Canada.
Ther Drug Monit. 1989;11(3):354-60. doi: 10.1097/00007691-198905000-00022.
A new sensitive analytical method is described for the quantitation of fluphenazine in 1 ml plasma samples after low oral doses of this antipsychotic agent. The drug is isolated by a simple one step extraction technique and analyzed by high-performance liquid chromatography (HPLC) with coulometric detection. The limit of detection for fluphenazine was 10 pg/ml of plasma and standard curves from 25 to 1,000 pg/ml were linear with an overall coefficient of variation (CV) of less than 5%. The lowest quantifiable concentration of 25 pg/ml could be determined with a CV of 4.7%. The sensitivity of the HPLC assay was such that plasma concentrations of fluphenazine could be followed for 2 days following administration of a single 10 mg oral dose of fluphenazine dihydrochloride to healthy volunteers. Known metabolites of fluphenazine did not interfere in the assay as they eluted with retention times different from that of fluphenazine.
本文描述了一种新的灵敏分析方法，用于在口服低剂量这种抗精神病药物后，对1毫升血浆样本中的氟奋乃静进行定量分析。该药物通过简单的一步萃取技术分离，并采用库仑检测的高效液相色谱法（HPLC）进行分析。氟奋乃静的检测限为血浆10皮克/毫升，25至1000皮克/毫升的标准曲线呈线性，总变异系数（CV）小于5%。最低可定量浓度为25皮克/毫升，其CV为4.7%。HPLC测定法的灵敏度足以在健康志愿者单次口服10毫克盐酸氟奋乃静后，跟踪氟奋乃静的血浆浓度达2天。氟奋乃静的已知代谢物不会干扰该测定，因为它们的保留时间与氟奋乃静不同，会在不同时间洗脱。
