Midha K K, Cooper J K, Hawes E M, Hubbard J W, Korchinski E D, McKay G
College of Pharmacy, University of Saskatchewan, Saskatoon, Canada.
Ther Drug Monit. 1988;10(2):177-83. doi: 10.1097/00007691-198802000-00011.
A new analytical method has been developed for the simultaneous quantitation of haloperidol and reduced haloperidol in plasma. The method is based on high performance liquid chromatography (HPLC) with coulometric detection. The extraction and sample clean up procedures are simple and rapid to execute, yet yield chromatograms virtually free of interference from endogenous plasma constituents, such that the extraordinary sensitivity of the coulometric detector can be exploited fully. The detection limits for haloperidol and reduced haloperidol are 20 pg/ml plasma, and the limits of quantitation are 50 pg/ml for both drug and metabolite. Standard curves were linear down to 50 pg/ml with coefficients of variation of less than 7.0% at the limits of quantitation. The method was applied to the study of the plasma levels of haloperidol and reduced haloperidol in two healthy subjects. It was possible to monitor the plasma levels of haloperidol for at least 96 h (4 days) after the administration of a 5-mg oral dose of haloperidol. It was also possible to monitor reduced haloperidol levels over 96 h in one subject, although the metabolite was not detectable in the plasma of the other at any stage.
已开发出一种新的分析方法，用于同时定量血浆中的氟哌啶醇和还原型氟哌啶醇。该方法基于高效液相色谱法（HPLC）和库仑检测。提取和样品净化程序执行简单且快速，但产生的色谱图几乎不受内源性血浆成分的干扰，从而可以充分利用库仑检测器的非凡灵敏度。氟哌啶醇和还原型氟哌啶醇的检测限为血浆20 pg/ml，定量限均为50 pg/ml。标准曲线在50 pg/ml以下呈线性，在定量限处变异系数小于7.0%。该方法应用于两名健康受试者氟哌啶醇和还原型氟哌啶醇血浆水平的研究。口服5 mg氟哌啶醇后，有可能至少96小时（4天）监测氟哌啶醇的血浆水平。在一名受试者中，也有可能在96小时内监测还原型氟哌啶醇水平，尽管在另一名受试者的血浆中任何阶段都未检测到该代谢物。
