Tkachenko E K, Barabash R D, Vovchuk S V
Ukr Biokhim Zh (1978). 1989 Mar-Apr;61(2):44-9.
Optimal conditions are determined for isolation and purification of peroxidase (EC 1.11.1.7) from the mixed human saliva by gel-filtration and ion chromatography. Such properties as pH and temperature optima, molecular weight, Km, amino acid composition, the effect of activators and inhibitors (purified saliva peroxidases and commercial preparation of horse-radish peroxidase "Reanal", Hungary) are studied with the use of different substrates. The data obtained are necessary to optimize production processes of isolation, storage and methods of the experimental and clinical application of peroxidases of different origin.
通过凝胶过滤和离子色谱法,确定了从混合人唾液中分离和纯化过氧化物酶(EC 1.11.1.7)的最佳条件。使用不同底物研究了诸如最适pH和温度、分子量、米氏常数、氨基酸组成、激活剂和抑制剂的作用(纯化的唾液过氧化物酶和匈牙利“Reanal”辣根过氧化物酶商业制剂)等性质。所获得的数据对于优化不同来源过氧化物酶的分离、储存生产工艺以及实验和临床应用方法是必要的。