[Effect of horse radish peroxidase immobilization on the kinetics of enzymatic oxidation of guaiacol in frozen solutions].

The kinetics of horse radish peroxidase (EC 1.11.1.7) catalyzed oxidation of guaiacol by hydrogen peroxide has been studied within the temperature region of +20--35 degrees C. The covalent attachment of the enzyme to polyacrylamide gel had no significant influence on the activation energy of the reaction in liquid solutions. For frozen solutions it was demonstrated that the temperature dependencies of the reaction rate differ greatly for the soluble and bound forms of the enzyme. The observed phenomenon is probably due to the differences in the molecular mobility, distribution and arrangement of reactants in frozen state.