精子运动参数仪：单头种猪精子运动性的电学特征。

Spermometer: electrical characterization of single boar sperm motility.

机构信息

BIOS - Lab on a Chip Group, MESA+ Institute for Nanotechnology and MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, the Netherlands.

BIOS - Lab on a Chip Group, MESA+ Institute for Nanotechnology and MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, the Netherlands; Department of Obstetrics and Gynaecology, Radboud University Nijmegen Medical Centre, Nijmegen, the Netherlands.

出版信息

Fertil Steril. 2016 Sep 1;106(3):773-780.e6. doi: 10.1016/j.fertnstert.2016.05.008. Epub 2016 Jun 6.

DOI:10.1016/j.fertnstert.2016.05.008

PMID:27281780

Abstract

OBJECTIVE

To study single sperm boar motility using electrical impedance measurements in a microfluidic system.

DESIGN

Comparison of the optical data and electrical impedance data.

SETTING

Research laboratory at a university.

ANIMAL(S): Boar semen sample were used.

INTERVENTION(S): A microfluidic system is developed that is able to spatially confine single boar sperm cells and allows noninvasive analysis of their motility on the single cell level. Using this system, the single sperm motility was affected by changing the temperature or adding chemical stimuli (caffeine). The retrieved electrical impedance and video data were processed using Matlab.

MAIN OUTCOME MEASURE(S): The sperm beat frequency and amplitude determined from the electrical impedance and video data.

RESULT(S): The electrically measured sperm beat frequency was verified by optical analysis and in correspondence. Furthermore the microfluidic platform allowed single sperm analysis by altering the sperm by temperature and chemical stimuli.

CONCLUSION(S): This platform could be exploited as a potential tool to study sperm cells on the single cell level and to perform advanced sperm selection for intracytoplasmic sperm injection (ICSI) applications.

摘要