de Bruijn Douwe S, Van de Waal Dedmer B, Helmsing Nico R, Olthuis Wouter, van den Berg Albert
BIOS Lab-on-a-Chip group MESA+ Institute for Nanotechnology Max Planck-University of Twente Center for Complex Fluid Dynamics University of Twente Drienerlolaan 5 Enschede Overijssel 7522 NB The Netherlands.
Department of Aquatic Ecology Netherlands Institute of Ecology (NIOO-KNAW) Droevendaalsesteeg 10 Wageningen 6708 PB The Netherlands.
Glob Chall. 2022 Dec 7;7(3):2200151. doi: 10.1002/gch2.202200151. eCollection 2023 Mar.
Calcifying algae, like coccolithophores, greatly contribute to the oceanic carbon cycle and are therefore of particular interest for ocean carbon models. They play a key role in two processes that are important for the effective CO flux: The organic carbon pump (photosynthesis) and the inorganic carbon pump (calcification). The relative contribution of calcification and photosynthesis can be measured in algae by the amount of particulate inorganic carbon (PIC) and particulate organic carbon (POC). A microfluidic impedance cytometer is presented, enabling non-invasive and high-throughput assessment of the calcification state of single coccolithophore cells. Gradual modification of the exoskeleton by acidification results in a strong linear fit ( = 0.98) between the average electrical phase and the PIC:POC ratio of the coccolithophore 920/9. The effect of different CO treatments on the PIC:POC ratio, however, is inconclusive, indicating that there is no strong effect observed for this particular strain. Lower PIC:POC ratios in cultures that grew to higher cell densities are found, which are also recorded with the impedance-based PIC:POC sensor. The development of this new quantification tool for small volumes paves the way for high-throughput analysis while applying multi-variable environmental stressors to support projections of the future marine carbon cycle.
钙化藻类，如颗石藻，对海洋碳循环有很大贡献，因此对于海洋碳模型来说具有特别的研究价值。它们在对有效碳通量很重要的两个过程中起着关键作用：有机碳泵（光合作用）和无机碳泵（钙化作用）。钙化作用和光合作用的相对贡献可以通过藻类中颗粒无机碳（PIC）和颗粒有机碳（POC）的含量来衡量。本文介绍了一种微流控阻抗细胞仪，它能够对单个颗石藻细胞的钙化状态进行非侵入性的高通量评估。通过酸化对外骨骼进行逐步改性，导致平均电相位与颗石藻920/9的PIC:POC比率之间呈现出很强的线性拟合（R² = 0.98）。然而，不同二氧化碳处理对PIC:POC比率的影响尚无定论，这表明对于这个特定菌株没有观察到明显的影响。在生长到更高细胞密度的培养物中发现了较低的PIC:POC比率，基于阻抗的PIC:POC传感器也记录到了这一点。这种用于小体积样品的新型定量工具的开发，为在应用多变量环境压力因素以支持未来海洋碳循环预测时进行高通量分析铺平了道路。
