Hu Jimeng, Yang Tian, Xu Hua, Hu Mengbo, Wen Hui, Jiang Haowen
Department of Urology, Huashan Hospital, Fudan University, No. 12 WuLuMuQi Middle Road, Shanghai, 200040, People's Republic of China.
Int Urol Nephrol. 2016 Oct;48(10):1649-55. doi: 10.1007/s11255-016-1341-9. Epub 2016 Jun 9.
We aimed to investigate whether icaritin (ICT) would inhibit serum proinflammatory cytokines and postpone prostate cancer (PCa) development and progression in both normal diet and high-fat diet (HFD) transgenic adenocarcinoma mouse prostate (TRAMP) mice.
TRAMP mice were randomly divided into four groups: normal diet with/without ICT group and HFD with/without ICT group. Each TRAMP mouse received intraperitoneal injection of ICT solution at the dose of 30 mg/kg 5 times per week.
ICT treatment could significantly increase the survival when compared with those in normal diet group (P = 0.015, log-rank test) and HFD group (P = 0.009, log-rank test). Proinflammatory cytokine levels, including IL-1α, IL-1β, IL-6, and TNF-α, were decreased more or less in ICT-treated TRAMP mice. Moreover, significant higher inflammation scores were detected in normal diet group and HFD group compared with their relevant ICT treatment groups (P = 0.026 and P = 0.006, respectively). Meanwhile, the incidences of well-differentiated tumor tissue in two ICT treatment groups (39.13 and 31.82 %) were moderately higher than control groups (29.41 and 20.00 %, respectively), though no significant difference was observed.
Taken together, our findings indicate that ICT could inhibit the development and progression of PCa in TRAMP mice via inhibiting proinflammatory cytokines.
我们旨在研究淫羊藿素（ICT）是否会抑制血清促炎细胞因子，并延缓正常饮食和高脂饮食（HFD）的转基因腺癌小鼠前列腺（TRAMP）小鼠的前列腺癌（PCa）发展和进展。
将TRAMP小鼠随机分为四组：正常饮食有/无ICT组和高脂饮食有/无ICT组。每只TRAMP小鼠每周接受5次腹腔注射剂量为30mg/kg的ICT溶液。
与正常饮食组（P = 0.015，对数秩检验）和高脂饮食组（P = 0.009，对数秩检验）相比，ICT治疗可显著提高生存率。在接受ICT治疗的TRAMP小鼠中，促炎细胞因子水平，包括IL-1α、IL-1β、IL-6和TNF-α，或多或少有所降低。此外，与各自的ICT治疗组相比，正常饮食组和高脂饮食组的炎症评分显著更高（分别为P = 0.026和P = 0.006）。同时，两个ICT治疗组中高分化肿瘤组织的发生率（分别为39.13%和31.82%）略高于对照组（分别为29.41%和20.00%），尽管未观察到显著差异。
综上所述，我们的研究结果表明，ICT可通过抑制促炎细胞因子来抑制TRAMP小鼠中PCa的发展和进展。
