胰岛素样生长因子1相关通路与高脂饮食促进转基因腺癌小鼠前列腺（TRAMP）癌进展
Insulin-Like growth factor 1 related pathways and high-fat diet promotion of transgenic adenocarcinoma mouse prostate (TRAMP) cancer progression.
作者信息
Xu H, Jiang H W, Ding Q
机构信息
Departamento de Urología, Hospital Huashan, Universidad Fudan, Shanghai, PR China.
Departamento de Urología, Hospital Huashan, Universidad Fudan, Shanghai, PR China.
出版信息
Actas Urol Esp. 2015 Apr;39(3):161-8. doi: 10.1016/j.acuro.2014.05.014. Epub 2014 Oct 28.
INTRODUCTION
We aimed to investigate the role of IGF-1 related pathway in high-fat diet (HFD) promotion of TRAMP mouse PCa progression.
METHODS
TRAMP mice were randomly divided into two groups: HFD group and normal diet group. TRAMP mice of both groups were sacrificed and sampled on the 20th, 24th and 28th week respectively. Serum levels of insulin, IGF-1 and IGF-2 were tested by ELISA. Prostate tissue of TRAMP mice was used for both HE staining and immunohistochemical staining of IGF-1 related pathway proteins, including IGF-1Rα, IGF -1Rβ, IGFBPs and AKT.
RESULTS
The mortality of TRAMP mice from HFD group was significantly higher than that of normal diet group (23.81% and 7.14%, p=.035). The tumor incidence of HFD TRAMP mice at 20(th) week was significantly higher than normal diet group (78.57% and 35.71%, p=.022). Serum IGF-1 level of HFD TRAMP mice was significantly higher than that of normal diet TRAMP mice. Serum IGF-1 level tended to increase with HFD TRAMP mice's age. HFD TRAMP mice had higher positive staining rate of IGF-1Rα, IGF-1Rβ, IGFBP3 and Akt than normal diet TRAMP mice.
CONCLUSIONS
IGF-1 related pathway played an important role in high-fat diet promotion of TRAMP mouse PCa development and progression.
引言
我们旨在研究胰岛素样生长因子-1（IGF-1）相关通路在高脂饮食（HFD）促进TRAMP小鼠前列腺癌进展中的作用。
方法
将TRAMP小鼠随机分为两组：高脂饮食组和正常饮食组。两组TRAMP小鼠分别在第20、24和28周处死并取样。采用酶联免疫吸附测定（ELISA）法检测血清胰岛素、IGF-1和IGF-2水平。将TRAMP小鼠的前列腺组织用于苏木精-伊红（HE）染色以及IGF-1相关通路蛋白的免疫组化染色，这些蛋白包括IGF-1Rα、IGF-1Rβ、胰岛素样生长因子结合蛋白（IGFBPs）和蛋白激酶B（AKT）。
结果
高脂饮食组TRAMP小鼠的死亡率显著高于正常饮食组（分别为23.81%和7.14%，p = 0.035）。高脂饮食的TRAMP小鼠在第20周时的肿瘤发生率显著高于正常饮食组（分别为78.57%和35.71%，p = 0.022）。高脂饮食的TRAMP小鼠血清IGF-1水平显著高于正常饮食的TRAMP小鼠。高脂饮食的TRAMP小鼠血清IGF-1水平随年龄增长呈上升趋势。高脂饮食的TRAMP小鼠的IGF-1Rα、IGF-1Rβ、IGFBP3和Akt的阳性染色率高于正常饮食的TRAMP小鼠。
结论
IGF-1相关通路在高脂饮食促进TRAMP小鼠前列腺癌发生和进展中起重要作用。
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