Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University , 1-1 Minamiohsawa, Hachioji, Tokyo 192-0397, Japan.
Anal Chem. 2016 Jun 21;88(12):6135-9. doi: 10.1021/acs.analchem.6b01566. Epub 2016 Jun 13.
A fluorescence enhanced phenomenon was found within a micrometer-sized liquid droplet, and it was adopted to construct droplet enhanced fluorescence (DEF) for ultrasensitive fluorescence detection. In this paper, an inkjet was utilized to eject perfect spherical droplets to construct a microspherical resonator and to develop a DEF system. It was utilized to implement ultrasensitive fluorescence detection in a liquid specimen with a volume of several microliters. The DEF detection of fluorescent molecules, fluorescein sodium, was used as a model to validate the proposed enhanced fluorescence detection method. A low limit of detection (LOD) for fluorescein sodium of 124 pM was obtained. The sensitive detection of single stranded DNA (ssDNA) was experimentally completed, with a wide range of linearity with a LOD of 312 pM. The proposed mechanism can be used as an ultrasensitive detection technique for analyzing microliters of liquid samples.
在微米级液滴中发现了荧光增强现象，并采用该现象构建了液滴增强荧光（DEF）以进行超灵敏荧光检测。本文利用喷墨技术喷射出完美的球形液滴，构建了微球谐振器并开发了 DEF 系统。该系统用于对几微升液体样本中的超灵敏荧光进行检测。以荧光素钠作为荧光分子的 DEF 检测为例，验证了所提出的增强荧光检测方法。获得了荧光素钠的低检测限（LOD）为 124 pM。实验完成了单链 DNA（ssDNA）的灵敏检测，线性范围很宽，LOD 为 312 pM。所提出的机制可用作分析微升液体样品的超灵敏检测技术。
