Adams J H, Shiels I A, de Vos A J
Department of Parasitology, University of Queensland, St. Lucia, Australia.
Vet Parasitol. 1989 Apr;31(1):7-12. doi: 10.1016/0304-4017(89)90003-4.
Antigens of Anaplasma centrale, Onderstepoort isolate, and A. marginale, Wacol isolate, were analysed by a Western blotting technique. Sera from A. centrale-infected calves reacted to 41- and 38-kDa antigens in A. centrale and a 41-kDa antigen in A. marginale. Serum collected during the primary reaction from an A. marginale-infected calf reacted only to the 41-kDa antigen of A. marginale; heterologous antibody response to the 41-kDa antigen of A. centrale did occur later during the infection, but remained markedly weaker than the homologous response. The serologic cross-reactivity to this 41-kDa Anaplasma antigen confirms that it is common to the genus and also that it is a heterogeneous complex.
采用蛋白质印迹技术分析了中央无形体翁德斯特普特分离株和边缘无形体瓦科尔分离株的抗原。来自感染中央无形体的犊牛的血清与中央无形体中的41 kDa和38 kDa抗原以及边缘无形体中的41 kDa抗原发生反应。在初次反应期间从感染边缘无形体的犊牛采集的血清仅与边缘无形体的41 kDa抗原发生反应；在感染后期确实出现了对中央无形体41 kDa抗原的异源抗体反应，但仍明显弱于同源反应。对这种41 kDa无形体抗原的血清学交叉反应证实，它是该属共有的，也是一种异质复合物。
