观察枯草芽孢杆菌营养生长和孢子形成过程

Visualizing Bacillus subtilis During Vegetative Growth and Spore Formation.

作者信息

Wang Xindan, Montero Llopis Paula

机构信息

Department of Microbiology & Immunobiology, Harvard Medical School, HIM Building 1018, 77 Ave Louis Pasteur, Boston, MA, 02115, USA.

出版信息

Methods Mol Biol. 2016;1431:275-87. doi: 10.1007/978-1-4939-3631-1_19.

DOI:10.1007/978-1-4939-3631-1_19

PMID:27283315

Abstract

Bacillus subtilis is the most commonly used Gram-positive bacterium to study cellular processes because of its genetic tractability. In addition, during nutrient limitation, B. subtilis undergoes the development process of spore formation, which is among the simplest examples of cellular differentiation. Many aspects of these processes have benefited from fluorescence microscopy. Here, we describe basic wide-field fluorescence microscopy techniques to visualize B. subtilis during vegetative growth, and the developmental process of sporulation.

摘要

枯草芽孢杆菌因其遗传易处理性，是研究细胞过程最常用的革兰氏阳性细菌。此外，在营养限制期间，枯草芽孢杆菌会经历孢子形成的发育过程，这是细胞分化最简单的例子之一。这些过程的许多方面都受益于荧光显微镜技术。在这里，我们描述基本的宽场荧光显微镜技术，以观察枯草芽孢杆菌在营养生长期间的情况，以及孢子形成的发育过程。