Shorokhova A P, Suzina N E, Polivtseva V N, Esikova T Z, Ross D V, Kholodenko V P, Abashina T N, Duda V I, Boronin A M
Mikrobiologiia. 2017 Jan-Feb;86(1):39-46.
The effect of ultramicrobacterial epibionts of the genera Kaistia (strain NF1), Chryseobacterium (strain NF4), and Stenotrophomonas (strain FM3) on the process of sporulation of Bacillus subtilis ATCC 6633 was studied. The investigated strains of ultramicrobacteria (UMB) were found to inhibit the sporulation process of B. subtilis ATCC 6633 in binary mixed cultures, exhibiting a 3-day delay of the onset of sporulation compared to the control one, an extended period of the prospore maturation, formation of the fraction of immature spores, and development of ultrastructural defects in many endospores. Thus, investigation of binary mixed cultures of B. subtilis and UMB revealed that, apart from suppression of reproduction and lysis of host vegetative cells, inhibition of spore formation and destruction of endospores was yet another feature of intermicrobial parasitism. The UMB parasites of the studied genera are assumed to participate in the regulation of development and reproduction of B. subtilis in natural habitats of this spore-forming bacterium.
研究了凯氏菌属（菌株NF1）、金黄杆菌属（菌株NF4）和嗜麦芽窄食单胞菌属（菌株FM3）的超微细菌外共生体对枯草芽孢杆菌ATCC 6633芽孢形成过程的影响。在二元混合培养物中，所研究的超微细菌（UMB）菌株被发现抑制枯草芽孢杆菌ATCC 6633的芽孢形成过程，与对照相比，芽孢形成开始延迟3天，前芽孢成熟时间延长，形成未成熟孢子部分，并且许多内生孢子出现超微结构缺陷。因此，对枯草芽孢杆菌和UMB二元混合培养物的研究表明，除了抑制宿主营养细胞的繁殖和裂解外，抑制孢子形成和破坏内生孢子是微生物间寄生的另一个特征。假定所研究属的UMB寄生虫参与在这种产芽孢细菌的自然栖息地中对枯草芽孢杆菌发育和繁殖的调节。
