表型芯片™系统在真菌功能多样性和分解代谢多功能性研究中的应用

Phenotype MicroArray™ system in the study of fungal functional diversity and catabolic versatility.

作者信息

Pinzari Flavia, Ceci Andrea, Abu-Samra Nadir, Canfora Loredana, Maggi Oriana, Persiani Annamaria

机构信息

Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia agraria, Centro di Ricerca per lo Studio delle Relazioni tra Pianta e Suolo (CREA-RPS), Via della Navicella 2-4, 00184 Rome, Italy; Natural History Museum, Life Sciences Department, Cromwell Road, London SW7 5BD, UK.

Dipartimento di Biologia ambientale, Sapienza Università di Roma, P.le Aldo Moro, 00185 Rome, Italy.

出版信息

Res Microbiol. 2016 Nov-Dec;167(9-10):710-722. doi: 10.1016/j.resmic.2016.05.008. Epub 2016 Jun 6.

DOI:10.1016/j.resmic.2016.05.008

PMID:27283363

Abstract

Fungi cover a range of important ecological functions associated with nutrient and carbon cycling in leaf litter and soil. As a result, research on existing relationships between fungal functional diversity, decomposition rates and competition is of key interest. Indeed, availability of nutrients in soil is largely the consequence of organic matter degradation dynamics. The Biolog Phenotype MicroArrays™ (PM) system allows for the testing of fungi against many different carbon sources at any one time. The use and potential of the PM system as a tool for studying niche overlap and catabolic versatility of saprotrophic fungi is discussed here, and examples of its application are provided.

摘要

真菌在凋落物和土壤中的养分及碳循环方面发挥着一系列重要的生态功能。因此，研究真菌功能多样性、分解速率和竞争之间的现有关系备受关注。事实上，土壤中养分的可利用性很大程度上是有机质降解动态的结果。Biolog表型微阵列™（PM）系统能够同时针对多种不同碳源对真菌进行测试。本文讨论了PM系统作为研究腐生真菌生态位重叠和分解代谢多样性工具的用途及潜力，并提供了其应用实例。