Mateos-Vivas María, Rodríguez-Gonzalo Encarnación, Domínguez-Álvarez Javier, García-Gómez Diego, Carabias-Martínez Rita
Department of Analytical Chemistry, Nutrition and Food Science, University of Salamanca, 37008 Salamanca, Spain.
Food Chem. 2016 Nov 15;211:827-35. doi: 10.1016/j.foodchem.2016.05.091. Epub 2016 May 14.
In this work we propose a rapid and efficient method for the joint determination of nucleosides and nucleotides in dairy and non-dairy baby foods based on hydrophilic interaction chromatography coupled to tandem mass spectrometry in the presence of diethylammonium (DEA) as a hydrophilic ion-pairing reagent (IP-HILIC-MS/MS). Sample treatment of the baby food included dilution with water and centrifugal ultrafiltration (CUF) with an additional washing step that notably improved the global performance of the process. Later dilution of the extract with acetonitrile allowed adequate separation in the HILIC system. With the proposed treatment, we obtained extraction recoveries higher than 80% and, additionally, no matrix effects were observed. The CUF-IP-HILIC-MS/MS method was validated according to the 2002/657/EC decision and was used for the quantification of nucleotides and nucleosides in sixteen samples of commercial baby foods.
在本研究中，我们提出了一种快速高效的方法，用于在作为亲水离子对试剂（IP-HILIC-MS/MS）的二乙铵（DEA）存在下，基于亲水相互作用色谱-串联质谱法联合测定乳制品和非乳制品婴儿食品中的核苷和核苷酸。婴儿食品的样品处理包括用水稀释和离心超滤（CUF），并增加了一个洗涤步骤，这显著提高了该过程的整体性能。随后用乙腈稀释提取物，可在亲水相互作用色谱系统中实现充分分离。采用所提出的处理方法，我们获得了高于80%的提取回收率，此外，未观察到基质效应。CUF-IP-HILIC-MS/MS方法根据2002/657/EC决议进行了验证，并用于定量分析16种市售婴儿食品样品中的核苷酸和核苷。
