Mateos-Vivas María, Domínguez-Álvarez Javier, Rodríguez-Gonzalo Encarnación, Carabias-Martínez Rita
Department of Analytical Chemistry, Nutrition and Food Science, University of Salamanca, 37008 Salamanca, Spain.
Food Chem. 2017 Oct 15;233:38-44. doi: 10.1016/j.foodchem.2017.04.060. Epub 2017 Apr 13.
The present work describes a method for the simultaneous determination of unmodified nucleosides and nucleotide mono-, di- and tri-phosphates by capillary electrophoresis coupled to mass spectrometry (CE-MS). The use of hexafluoro-2-propanol (HFIP) in the separation medium, and as an additive to the sheath liquid of the electrospray interface (ESI), generated a highly efficient and sensitive method. Instrumental limits of detection in the range of 14-53ngmL for nucleosides and 7-23, 20-49 and 64-124ngmL for nucleotide mono-, di-, and tri-phosphates, respectively, were found. Sample treatment involved diluting an aliquot of baby food with ultra-high quality water and applying centrifugation-assisted ultrafiltration (CUF). The proposed method was validated and used to analyse a variety of baby food samples (16 in total) such as fish, meat, fruits, and baby dairy desserts that may endogenously contain these analytes.
本研究描述了一种通过毛细管电泳-质谱联用（CE-MS）同时测定未修饰核苷以及核苷酸单磷酸、二磷酸和三磷酸的方法。在分离介质中使用六氟-2-丙醇（HFIP），并将其作为电喷雾接口（ESI）鞘液的添加剂，从而产生了一种高效且灵敏的方法。发现核苷的仪器检测限在14 - 53 ng/mL范围内，核苷酸单磷酸、二磷酸和三磷酸的检测限分别在7 - 23 ng/mL、20 - 49 ng/mL和64 - 124 ng/mL范围内。样品处理包括用超纯水稀释一份婴儿食品等分试样，并进行离心辅助超滤（CUF）。所提出的方法经过验证，并用于分析各种可能内源性含有这些分析物的婴儿食品样品（总共16种），如鱼类、肉类、水果和婴儿奶制品甜点。
