School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi'an, 710021, China; Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China.
School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi'an, 710021, China.
J Pharm Biomed Anal. 2019 Oct 25;175:112787. doi: 10.1016/j.jpba.2019.112787. Epub 2019 Jul 24.
Cordyceps sinensis is a traditional Chinese food. A high-throughput screening and quantitative method based on hydrophilic interaction chromatography coupled to quadrupole/Orbitrap high resolution mass spectrometry has been proposed for the joint measurement of nucleosides and their corresponding nucleotides in thirty-two Cordyceps sinensis samples. A total of thirty-two natural samples from six regions were enrolled. A multiple-step analysis strategy, including features extraction, noise level evaluation, mass shift correction and suspect spectral library spectra searching, was employed to discover, identify and validate the nucleosides and nucleotides. The remaining unknown compounds were identified with the characteristic structural fragments. The on-line dispersive solid-phase extraction system eliminates human-based steps errors and affords superior method reproducibility in the presence of hexafluoro-2-propanol and diethylamine as hydrophilic ion-pairing reagents. In view of the great difference of the quality, this high coverage confirmation strategy is of great reference value to the future quantitative analysis of potential nucleosides and nucleotides. Nucleosides and nucleotides ingredients could be considered as origins and quality assessment index of C. sinensis.
冬虫夏草是一种传统的中国食品。提出了一种基于亲水作用色谱-四极杆/轨道阱高分辨质谱联用的高通量筛选和定量方法，用于同时测定三十二种冬虫夏草样品中的核苷及其相应的核苷酸。共采集了来自六个地区的三十二个天然样本。采用多步分析策略，包括特征提取、噪声水平评估、质量位移校正和可疑光谱库光谱搜索，用于发现、鉴定和验证核苷和核苷酸。用特征结构片段鉴定其余未知化合物。在线分散固相萃取系统消除了人为步骤的误差，并在六氟-2-丙醇和二乙胺作为亲水离子对试剂存在的情况下提供了更好的方法重现性。鉴于质量的巨大差异，这种高覆盖率确认策略对未来潜在核苷和核苷酸的定量分析具有重要的参考价值。核苷和核苷酸成分可以被认为是冬虫夏草的来源和质量评估指标。
