Liu Bin, Wang Pan-Feng, Chai Jie, Hu Xiang-Quan, Gao Tingting, Chao Jian-Bin, Chen Ting-Gui, Yang Bin-Sheng
Institute of Molecular Science, Key Laboratory of Chemical Biology of Molecular Engineering of Education Ministry, Shanxi University, Taiyuan 030006, China.
Institute of Molecular Science, Key Laboratory of Chemical Biology of Molecular Engineering of Education Ministry, Shanxi University, Taiyuan 030006, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2016 Nov 5;168:98-103. doi: 10.1016/j.saa.2016.06.002. Epub 2016 Jun 3.
Three naphthol Schiff base-type fluorescent sensors, 1,3-Bis(2-hydroxy-1-naphthylideneamino)propane (L1), 1,3-Bis(1-naphthylideneamino)-2-hydroxypropane (L2) and 1,3-Bis(2-hydroxy-1-naphthylideneamino)-2-hydroxypropane (L3), have been synthesized. Their recognition abilities for Al(3+) are studied by fluorescence spectra. Coordination with Al(3+) inhibited the CN isomerization of Schiff base which intensely increase the fluorescence of L1-L3. Possessing a suitable space coordination structure, L3 is a best selective probe for Al(3+) over other metal ions in MeOH-HEPES buffer (3/7, V/V, pH=6.6, 25°C, λem=435nm). A turn-on ratio over 140-fold is triggered with the addition of 1.0 equiv. Al(3+) to L3. The binding constant Ka of L3-Al(3+) is found to be 1.01×10(6.5)M(-1) in a 1:1 complex mode. The detection limit for Al(3+) is 0.05μM. Theoretical calculations have also been included in support of the configuration of the L3-Al(3+) complex. Importantly, the probe L3 has been successfully used for fluorescence imaging in colon cancer SW480 cells.
已合成三种萘酚席夫碱型荧光传感器，即1,3 - 双（2 - 羟基 - 1 - 萘基亚氨基）丙烷（L1）、1,3 - 双（1 - 萘基亚氨基）-2 - 羟基丙烷（L2）和1,3 - 双（2 - 羟基 - 1 - 萘基亚氨基）-2 - 羟基丙烷（L3）。通过荧光光谱研究了它们对Al(3+)的识别能力。与Al(3+)配位抑制了席夫碱的CN异构化，这极大地增强了L1 - L3的荧光。L3具有合适的空间配位结构，是甲醇 - HEPES缓冲液（3/7，V/V，pH = 6.6，25°C，λem = 435nm）中对Al(3+)优于其他金属离子的最佳选择性探针。加入1.0当量的Al(3+)到L3中会引发超过140倍的开启比率。发现L3 - Al(3+)的结合常数Ka在1:1络合模式下为1.01×10(6.5)M(-1)。Al(3+)的检测限为0.05μM。还进行了理论计算以支持L3 - Al(3+)络合物的构型。重要的是，探针L3已成功用于结肠癌SW480细胞的荧光成像。
