Department of Chemical Biology and Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin 300070, PR China.
The Laboratory of Theoretical and Computational Chemistry, School of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Jan 15;207:31-38. doi: 10.1016/j.saa.2018.08.063. Epub 2018 Aug 31.
Developing high performance fluorescent chemo-sensors for in vitro and in vivo Al detection is highly desirable, because Al accumulation has been involved to various diseases. Herein, we report a highly selective and sensitive Schiff base fluorescent probe, HL, based on 2-hydroxynaphthalene, which can recognize aluminum ions and exhibit an "off-on" mode with high selectivity in methanol solutions. The detection limit of the probe for Al is as low as 10 M which was determined by fluorescent titration. The high selectivity and high sensitivity of HL for Al are attributed to the inhibition of ESIPT. Additionally, the distribution of intracellular Al ions could be observed under confocal fluorescence microscopy. Moreover, we also applied HL for in vivo detection of Al ions in living zebrafish larvae.
开发用于体外和体内 Al 检测的高性能荧光化学传感器是非常可取的，因为 Al 的积累与各种疾病有关。在此，我们报告了一种基于 2-羟基萘的高选择性和高灵敏度的席夫碱荧光探针 HL，它可以识别铝离子，并在甲醇溶液中表现出高选择性的“关闭-开启”模式。探针对 Al 的检测限低至 10 nM，这是通过荧光滴定法确定的。HL 对 Al 具有高选择性和高灵敏度归因于 ESIPT 的抑制。此外，还可以在共聚焦荧光显微镜下观察细胞内 Al 离子的分布。此外，我们还将 HL 用于活体斑马鱼幼虫体内 Al 离子的检测。
