设计具有 DNA 的聚电解质核壳来控制 TMPyP 结合。

Design of polyelectrolyte core-shells with DNA to control TMPyP binding.

机构信息

Centro de Química Estrutural, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais 1, 1049-001 Lisboa, Portugal; Unidade de Química Orgânica e Produtos Naturais, Departamento de Química, Universidade de Aveiro, Portugal.

Centro de Química Estrutural, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais 1, 1049-001 Lisboa, Portugal.

出版信息

Colloids Surf B Biointerfaces. 2016 Oct 1;146:127-35. doi: 10.1016/j.colsurfb.2016.05.047. Epub 2016 May 19.

DOI:10.1016/j.colsurfb.2016.05.047

PMID:27285535

Abstract

The interaction of DNA with 5,10,15,20-tetrakis(4-N-methylpyridiniumyl)porphyrin (TMPyP) in polyelectrolyte core-shells obtained via layer by layer adsorption of poly(sodium 4-styrenesulfonate), PSS, and poly(allylamine hydrochloride), PAH, polyelectrolytes was followed by steady state, time resolved fluorescence and by Fluorescence Lifetime Imaging Microscopy (FLIM). Our results show that DNA adsorption onto polyelectrolyte core-shell changes the TMPyP interaction within PSS/PAH core-shells structure and increase significantly the TMPyP uptake. Specific DNA/TMPyP interactions are also altered by DNA adsorption favouring porphyrin intercalation onto GC pair rich regions. Circular dichroism (CD) spectra reveal that DNA undergoes important conformational changes upon adsorption onto the core-shell surface, which are reverted upon TMPyP encapsulation.

摘要

通过层层吸附聚（对苯乙烯磺酸钠）（PSS）和聚（盐酸烯丙胺）（PAH）聚电解质，研究了 DNA 与 5,10,15,20-四（4-N-甲基吡啶基）卟啉（TMPyP）在聚电解质核壳中的相互作用。通过稳态、时间分辨荧光和荧光寿命成像显微镜（FLIM）进行了跟踪。我们的结果表明，DNA 吸附到聚电解质核壳上会改变 TMPyP 在 PSS/PAH 核壳结构内的相互作用，并显著增加 TMPyP 的摄取量。DNA 的吸附还通过有利于卟啉插入富含 GC 对的区域来改变 TMPyP 的特异性相互作用。圆二色（CD）光谱表明，DNA 在吸附到核壳表面时会发生重要的构象变化，而这些变化在 TMPyP 封装时会恢复。