Gärtner U, Gundert-Remy U, Stiehl A, Schulz T, Götz R, Walter B, Kommerell B
Department of Medicine, University of Heidelberg, F.R.G.
Z Gastroenterol. 1989 Apr;27(4):207-11.
Studies in rats by others indicated that sulfobromophthalein (BSP), bilirubin and indocyanine green are taken up by the liver and can be transported back to plasma against the prevailing concentration gradient (= countertransport). The present in vivo study was designed to determine whether the bile acids cholic acid and taurocholic acid under physiological conditions undergo appreciable countertransport as has been suggested by experiments in isolated hepatocytes. Experiments with BSP (controls) showed that injections of unlabeled BSP into rats five minutes after the administration of radiolabeled BSP was followed by a release of radioactivity into plasma (BSP-countertransport). In contrast bile acid countertransport could not be demonstrated, no matter whether it was tested 1, 5 or 8 minutes after the administration of radiolabeled cholic- or taurocholic acid.
其他针对大鼠的研究表明，磺溴酞钠（BSP）、胆红素和吲哚菁绿会被肝脏摄取，并能逆着当时的浓度梯度转运回血浆（即逆向转运）。本项体内研究旨在确定在生理条件下，胆酸和牛磺胆酸是否如在分离肝细胞实验中所提示的那样，会发生明显的逆向转运。用BSP进行的实验（对照组）显示，在给大鼠注射放射性标记的BSP五分钟后再注射未标记的BSP，随后放射性物质会释放到血浆中（BSP逆向转运）。相比之下，无论在注射放射性标记的胆酸或牛磺胆酸后1分钟、5分钟还是8分钟进行检测，均未证实存在胆汁酸逆向转运。
