Tsuji M, Kuwano E, Saito T, Eto M
a Department of Agricultural Chemistry , Kyushu University , Fukuoka 812 , Japan.
Biosci Biotechnol Biochem. 1992 Jan;56(5):778-82. doi: 10.1271/bbb.56.778.
A number of N-acyl-L-proline derivatives were synthesized and their biological activities were investigated by using lettuce (Lactuca sativa L. cv. Sacramento) seedling test. A wide variety of these compounds promoted root growth at 25°C both under light and in darkness. Of the compounds tested, N-(2-ftuorobenzoyl)-L-proline methyl ester (4) showed the highest activity and caused a 270% increase in the root elongation compared to the control. N-(2-Naphthoyl)-L-proline methyl ester (14) promoted the root growth, while N-(1-naphthoyl)-L-proline methyl ester inhibited it. L-Proline, benzoic acid, and 2-naphthoic acid had no significant effect on lettuce seedlings. Compounds 4 and 14, and N-(2-chlorobenzoyl)-L-proline methyl ester (7) reduced the inhibitory effect of 1 ppm ABA on the root growth, while the D-isomer of 4 was less activite than compound 4. Compounds 4, 7, and 14 did not show any rescue-activity for the complete inhibition of germination that was caused by treating 10 ppm of ABA.
合成了多种N-酰基-L-脯氨酸衍生物，并通过生菜（Lactuca sativa L. cv. Sacramento）幼苗试验研究了它们的生物活性。这些化合物中的多种在25°C下无论光照还是黑暗条件下均能促进根系生长。在所测试的化合物中，N-（2-氟苯甲酰基）-L-脯氨酸甲酯（4）表现出最高活性，与对照相比，根伸长增加了270%。N-（2-萘甲酰基）-L-脯氨酸甲酯（14）促进根系生长，而N-（1-萘甲酰基）-L-脯氨酸甲酯则抑制根系生长。L-脯氨酸、苯甲酸和2-萘甲酸对生菜幼苗没有显著影响。化合物4和14以及N-（2-氯苯甲酰基）-L-脯氨酸甲酯（7）降低了1 ppm脱落酸对根系生长的抑制作用，而化合物4的D-异构体活性低于化合物4。化合物4、7和14对10 ppm脱落酸处理导致的完全抑制发芽没有表现出任何挽救活性。
