Toxic and Hazardous Materials Branch, Environmental Research Laboratory, U.S. Environmental Protection Agency, 97333, Corvallis, OR, USA.
Environ Monit Assess. 1986 May;6(3):267-76. doi: 10.1007/BF00396794.
Lettuce (Lactuca sativa L., cv buttercrunch) was used to evaluate and compare the results from two different root elongation phytotoxicity test methods with the same six test substances. Seeds were either germinated in the dark on an inclined filter paper substrate with one end immersed in test solution or germinated in 0.1 strength nutrient solution with a 16 hr light period in a glass bulb mixed and aerated with compressed air. Sodium fluoride, monosodium methanearsonate and monuron reduced lettuce root length at similar concentrations for both methods, while cadmium chloride and 2,4-D root inhibition occurred at concentrations approximately one order of magnitude smaller in the solution culture method than in the substrate method. Two orders of magnitude difference were observed between the two methods for silver nitrate. The results indicate that the solution method is at least as sensitive for all the chemicals tested and much more sensitive for some.
生菜（Lactuca sativa L.，cv buttercrunch）被用于评估和比较两种不同的根伸长植物毒性测试方法的结果，这些方法使用了相同的六种测试物质。种子要么在黑暗中斜放在浸有测试溶液的滤纸基质上发芽，要么在 0.1 强度的营养溶液中发芽，16 小时光照周期在一个玻璃灯泡中，用压缩空气混合和充气。氟化钠、甲酸钠和敌草隆在两种方法中以相似的浓度降低了生菜根的长度，而氯化镉和 2,4-D 根抑制在溶液培养方法中的浓度比基质方法低约一个数量级。硝酸银在两种方法之间观察到两个数量级的差异。结果表明，对于所有测试的化学物质，溶液法至少同样敏感，对于某些化学物质则更为敏感。
