Spiegler Verena, Liebau Eva, Peppler Carolin, Raue Katharina, Werne Steffen, Strube Christina, Heckendorn Felix, Agyare Christian, Stark Timo, Hofmann Thomas, Hensel Andreas
Institute for Pharmaceutical Biology and Phytochemistry, University of Münster, Münster, Germany.
Department of Molecular Physiology, Institute for Zoophysiology, Münster, Germany.
Planta Med. 2016 Aug;82(13):1173-9. doi: 10.1055/s-0042-108209. Epub 2016 Jun 10.
Paullinia pinnata is a medicinal plant traditionally used in West Africa against a wide range of diseases including soil-transmitted helminthiases. In this study, a hydroethanolic root extract was investigated for its phytochemical composition and in vitro activity against the free-living nematode Caenorhabditis elegans as well as the larval stages of the parasitic helminths Ancylostoma caninum, Haemonchus contortus, Toxocara cati, and Trichuris vulpis.LC-MS analysis of the ethanol-water (1 : 1) extract revealed epicatechin and different A-type linked oligomeric and polymeric procyanidins as the predominant compounds.Within an in vitro mortality assay, the extract showed a lethal activity against T. cati (LC50 of 112 µg/mL), T. vulpis (LC50 of 17 µg/mL), and C. elegans (LC50 2.5 of mg/mL), but not against A. caninum. Additionally, effects on egg hatching and larval migration of H. contortus were investigated, but no inhibitory activity was observed.Overall, these findings rationalize the traditional use of the root extract from P. pinnata as an anthelmintic remedy and provide insight into the phytochemical composition of the extract.
羽叶番荔枝是一种药用植物，在西非传统上用于治疗多种疾病，包括土壤传播的蠕虫病。在本研究中，对其水乙醇根提取物的植物化学成分以及对自由生活线虫秀丽隐杆线虫和寄生蠕虫犬钩口线虫、捻转血矛线虫、猫弓首蛔虫和狐毛首线虫幼虫阶段的体外活性进行了研究。乙醇 - 水（1∶1）提取物的液相色谱 - 质谱分析表明，表儿茶素以及不同A型连接的低聚和聚合原花青素是主要化合物。在体外死亡率测定中，该提取物对猫弓首蛔虫（LC50为112μg/mL）、狐毛首线虫（LC50为17μg/mL）和秀丽隐杆线虫（LC50为2.5mg/mL）具有致死活性，但对犬钩口线虫没有致死活性。此外，还研究了对捻转血矛线虫卵孵化和幼虫迁移的影响，但未观察到抑制活性。总体而言，这些发现为羽叶番荔枝根提取物作为驱虫药物的传统用途提供了理论依据，并深入了解了该提取物的植物化学成分。
