CSIR-Institute of Genomics and Integrative Biology, Delhi University Campus, Mall Road, Delhi 110007, India.
CSIR-Indian Institute of Toxicology Research, M.G. Marg, Post Box No. 80, Lucknow 226001, U.P., India.
Eur J Pharm Biopharm. 2016 Aug;105:176-92. doi: 10.1016/j.ejpb.2016.06.002. Epub 2016 Jun 7.
Rapidly increasing malignant neoplastic disease demands immediate attention. Several dietary compounds have recently emerged as strong anti-cancerous agents. Among, Bromelain (BL), a protease from pineapple plant, was used to enhance its anti-cancerous efficacy using nanotechnology. In lieu of this, hyaluronic acid (HA) grafted PLGA copolymer, having tumor targeting ability, was developed. BL was encapsulated in copolymer to obtain BL-copolymer nanoparticles (NPs) that ranged between 140 to 281nm in size. NPs exhibited higher cellular uptake and cytotoxicity in cells with high CD44 expression as compared with non-targeted NPs. In vivo results on tumor bearing mice showed that NPs were efficient in suppressing the tumor growth. Hence, the formulation could be used as a self-targeting drug delivery cargo for the remission of cancer.
恶性肿瘤疾病的迅速增加需要引起重视。最近，一些膳食化合物已成为强有力的抗癌剂。其中，菠萝蛋白酶（BL）是一种来自菠萝植物的蛋白酶，已被用于通过纳米技术提高其抗癌效果。在此基础上，开发了具有肿瘤靶向能力的透明质酸（HA）接枝 PLGA 共聚物。将 BL 包封在共聚物中，得到 BL-共聚物纳米颗粒（NPs），其粒径在 140 至 281nm 之间。与非靶向 NPs 相比，NPs 在高表达 CD44 的细胞中具有更高的细胞摄取率和细胞毒性。荷瘤小鼠的体内实验结果表明，NPs 能有效抑制肿瘤生长。因此，该制剂可用作自靶向药物递送载体，以缓解癌症。
