Thurston S J, Saffer J D
Jackson Laboratory, Bar Harbor, Maine 04609.
Anal Biochem. 1989 Apr;178(1):41-2. doi: 10.1016/0003-2697(89)90353-9.
We describe a method for the direct visualization of nucleic acids on nylon membranes. Nylon is weakly fluorescent under short wave ultraviolet light allowing membrane-bound nucleic acids to be detected with a sensitivity of 10 ng. This procedure involves no staining or destaining of the gels prior to transfer, does not require duplicate sample lanes or blots, and does not interfere with transfer of the nucleic acid to the membrane or subsequent hybridization.
我们描述了一种在尼龙膜上直接可视化核酸的方法。尼龙在短波紫外线下呈弱荧光，可检测到膜结合核酸，灵敏度为10纳克。该方法在转移前无需对凝胶进行染色或脱色，不需要重复的样品泳道或印迹，并且不干扰核酸向膜的转移或后续杂交。
