Haines Alicia M, Tobe Shanan S, Kobus Hilton J, Linacre Adrian
School of Biological Sciences, Flinders University, South Australia, Australia.
Electrophoresis. 2015 Mar;36(6):941-4. doi: 10.1002/elps.201400496. Epub 2015 Feb 23.
Nucleic acid staining dyes are used for detecting nucleic acids in electrophoresis gels. Historically, the most common dye used for gel staining is ethidium bromide, however due to its toxicity and mutagenicity other dyes that are safer to the user and the environment are preferred. This Short Communication details the properties of dyes now available and their sensitivity for detection of DNA and their ability to permeate the cell membrane. It was found that GelRed™ was the most sensitive and safest dye to use with UV light excitation, and both GelGreen™ and Diamond™ Nucleic Acid Dye were sensitive and the safer dyes using blue light excitation.
核酸染色染料用于检测电泳凝胶中的核酸。从历史上看，用于凝胶染色最常用的染料是溴化乙锭，然而，由于其毒性和致突变性，现在更倾向于使用对使用者和环境更安全的其他染料。本短讯详细介绍了现有染料的特性、它们对DNA检测的灵敏度以及它们穿透细胞膜的能力。结果发现，GelRed™是在紫外光激发下使用最灵敏且最安全的染料，而GelGreen™和Diamond™核酸染料在蓝光激发下既灵敏又安全。
