Bayrakli Ismail, Turkmen Aysenur, Cem Kockar Muhammet
Suleyman Demirel University, Biomedical Engineering, Bati kampusu, Isparta, Turkey
Suleyman Demirel University, Biomedical Engineering, Bati kampusu, Isparta, Turkey.
Appl Spectrosc. 2016 Aug;70(8):1269-77. doi: 10.1177/0003702816654052. Epub 2016 Jun 13.
The purpose of this study is to assess the feasibility of using breath ammonia analysis based on off-axis cavity-enhanced absorption spectroscopy (OA-CEAS) with an external-cavity diode laser (ECL) for noninvasive, real-time diagnosis of Helicobacter pylori (HP) infection. Analyses are performed for the breath of 15 healthy volunteers, and eight children and 19 adults with HP infection. The range of ammonia levels for healthy participants is determined to be between 178 and 610 ppb, whereas the ranges for child and adult patients with HP infection are measured to be 457-2470 ppb and 450-2990 ppb, respectively. The ammonia concentrations for patients with HP infection are significantly higher than the concentrations for healthy volunteers. However, no sharp boundary between the ammonia concentrations in the breath of patients with HP infection and healthy volunteers is observed. No correlation between breath ammonia and either body mass index (BMI) or age is found. The reported results suggest that our breath ammonia measurement system has the potential for future use in easy, noninvasive diagnosis of HP infection.
本研究的目的是评估基于外腔二极管激光器（ECL）的离轴腔增强吸收光谱技术（OA-CEAS）进行呼出气氨分析用于无创、实时诊断幽门螺杆菌（HP）感染的可行性。对15名健康志愿者以及8名儿童和19名成年HP感染患者的呼气进行了分析。健康参与者的氨水平范围确定为178至610 ppb，而儿童和成年HP感染患者的氨水平范围分别测得为457 - 2470 ppb和450 - 2990 ppb。HP感染患者的氨浓度显著高于健康志愿者的浓度。然而，未观察到HP感染患者与健康志愿者呼出气中氨浓度之间存在明显界限。未发现呼出气氨与体重指数（BMI）或年龄之间存在相关性。报告结果表明，我们的呼出气氨测量系统具有未来用于简便、无创诊断HP感染的潜力。
