Buka D, Dvořák J, Sitorová V, Hátlová J, Richter I, Sirák I
Klin Onkol. 2016;29(3):204-9. doi: 10.14735/amko2016204.
The goal of this study is to examine the effect of neoadjuvant radiochemotherapy on the density of CD8(+) tumor infiltrating lymphocytes (TILs) in endoscopical biopsies and resection specimens from patients with rectal adenocarcinoma before and after therapy.
In total, 53 patients with locally advanced rectal cancer were studied.
The median density of CD8(+) TILs in pretreatment biopsies was 12 (1- 232) and that in surgical specimens after radiochemotherapy was 18 (1- 319). During radiochemotherapy, the density of CD8(+) TILs increased in 30 patients (57%), decreased in 18 (34%), and did not change in one. It was not possible to assess the dynamics of CD8(+) TILs density in four patients. The increased density of CD8(+) TILs after radiochemotherapy was associated with a median survival rate 2.5 times longer than that associated with no increase in density.
In the present study, the density of CD8(+) TILs in endoscopical biopsies before radiochemotherapy, the density in resection specimens after radiochemotherapy, or in changes in the density after radiochemotherapy showed no predictive or prognostic significance. However, studying a larger number of patients may show that CD8(+) TILs density is of predictive or prognostic significance.
本研究的目的是检测新辅助放化疗对直肠癌患者治疗前后内镜活检组织及手术切除标本中CD8(+)肿瘤浸润淋巴细胞（TILs）密度的影响。
共研究了53例局部晚期直肠癌患者。
放化疗前活检组织中CD8(+) TILs的中位密度为12（1 - 232），放化疗后手术标本中的中位密度为18（1 - 319）。在放化疗期间，30例患者（57%）的CD8(+) TILs密度增加，18例（34%）降低，1例无变化。4例患者无法评估CD8(+) TILs密度的动态变化。放化疗后CD8(+) TILs密度增加与中位生存率相关，其生存时间比密度未增加者长2.5倍。
在本研究中，放化疗前内镜活检组织中CD8(+) TILs的密度、放化疗后手术切除标本中的密度或放化疗后密度的变化均未显示出预测或预后意义。然而，研究更多患者可能会显示CD8(+) TILs密度具有预测或预后意义。
