Gamble Gary R, Berrang Mark E, Buhr R Jeff, Hinton Arthur, Bourassa Dianna V, Johnston John J, Ingram Kimberly D, Adams Eric S, Feldner Peggy W
U.S. National Poultry Research Center, Agricultural Research Service, U.S. Department of Agriculture, Athens, Georgia 30605, USA.
Office of Public Health Science, Food Safety and Inspection Service, U.S. Department of Agriculture, Building D, Suite 320, Fort Collins, Colorado 80526, USA.
J Food Prot. 2016 May;79(5):710-4. doi: 10.4315/0362-028X.JFP-15-461.
Numerous antimicrobial chemicals are currently utilized as processing aids with the aim of reducing pathogenic bacteria on processed poultry carcasses. Carryover of active sanitizer to a carcass rinse solution intended for recovery of viable pathogenic bacteria by regulatory agencies may cause false-negative results. This study was conducted to document the potential carryover effect of five sanitizing chemicals commonly used as poultry processing aids for broilers in a postchill dip. The effect of postdip drip time on the volume of sanitizer solution carryover was first determined by regression of data obtained from 10 carcasses. The five sanitizer solutions were diluted with buffered peptone water at 0-, 1-, and 5-min drip time equivalent volumes as determined by the regression analysis. These solutions were then spiked to 10(5) CFU/ml with a mixture of five nalidixic acid-resistant Salmonella enterica serovars, stored at 4°C for 24 h, and finally enumerated by plate count on brilliant green sulfa agar containing nalidixic acid. At the 0- and 1-min drip time equivalents, no Salmonella recovery was observed in three of the five sanitizers studied. At the 5-min drip time equivalent, one of these sanitizers still exhibited significant (P ≤ 0.05) bactericidal activity. These findings potentially indicate that the currently utilized protocol for the recovery of Salmonella bacteria from postchill sanitizer interventions may lead to false-negative results due to sanitizer carryover into the carcass rinsate.
目前，许多抗菌化学品被用作加工助剂，目的是减少加工禽体上的病原菌。活性消毒剂残留到监管机构用于回收活病原菌的胴体冲洗液中，可能会导致假阴性结果。本研究旨在记录五种常用于肉鸡加工后冷却浸蘸处理的消毒剂化学品的潜在残留效应。首先通过对10只禽体的数据进行回归分析，确定浸蘸后沥干时间对消毒剂溶液残留量的影响。根据回归分析确定的0分钟、1分钟和5分钟沥干时间的等效体积，用缓冲蛋白胨水稀释这五种消毒剂溶液。然后，用五种耐萘啶酸的肠炎沙门氏菌血清型混合物将这些溶液接种至10⁵CFU/ml，在4°C下储存24小时，最后在含有萘啶酸的亮绿磺胺琼脂平板上进行平板计数。在0分钟和1分钟沥干时间等效值时，在所研究的五种消毒剂中，有三种未观察到沙门氏菌的回收。在5分钟沥干时间等效值时，其中一种消毒剂仍表现出显著（P≤0.05）的杀菌活性。这些发现可能表明，目前用于从冷却后消毒剂干预措施中回收沙门氏菌的方案，可能会因消毒剂残留到胴体冲洗液中而导致假阴性结果。
