Hoskin P J, Alsayed-Omar O, Hanks G W, Johnston A, Turner P
Continuing Care Unit, Royal Marsden Hospital, Sutton, Surrey, UK.
Ann Clin Biochem. 1989 Mar;26 ( Pt 2):182-4. doi: 10.1177/000456328902600216.
The effect of six different methods of blood sample preparation on concentrations of morphine (M), morphine-3-glucuronide (M3G) and morphine-6-glucuronide (M6G) have been investigated using a specific high performance liquid chromatography assay. No difference between glass or plastic tubes was seen in concentrations of M, M3G, or M6G; and no difference between plasma and serum in M and M6G concentrations. M3G concentrations, however, were significantly lower in plasma compared with serum. Heparin had no effect on M, M6G or M3G, whereas citrate in a glass tube produced consistently lower concentrations of M, M3G and M6G. Standardization of sample collection using plasma samples in plastic heparin tubes is recommended for future studies.
使用特定的高效液相色谱法测定，研究了六种不同血样制备方法对吗啡（M）、吗啡 - 3 - 葡萄糖醛酸苷（M3G）和吗啡 - 6 - 葡萄糖醛酸苷（M6G）浓度的影响。在M、M3G或M6G的浓度方面，未观察到玻璃管和塑料管之间存在差异；在M和M6G的浓度方面，血浆和血清之间也没有差异。然而，与血清相比，血浆中的M3G浓度显著较低。肝素对M、M6G或M3G没有影响，而玻璃管中的柠檬酸盐会使M、M3G和M6G的浓度持续降低。建议在未来的研究中使用塑料肝素管中的血浆样本进行样本采集标准化。
