Oyama N, Yamauchi T, Yamane H, Murofushi N, Agatsuma M, Pour M, Mander L N
a Department of Applied Biological Chemistry , The University of Tokyo, Bunkyo-ku , Tokyo , 113 , Japan.
b Biotechnology Research Center , The University of Tokyo, Bunkyo-ku , Tokyo , 113 , Japan.
Biosci Biotechnol Biochem. 1996 Jan;60(2):305-8. doi: 10.1271/bbb.60.305.
Endogenous gibberellins (GAs) and GA-related compounds at two different developmental stages of apple seeds(Malus domestica cv. McIntosh) were analyzed by gas chromatography-mass spectrometry.From the seeds at ca. 10 and/or 14 weeks after full-bloom, the following GAs and 9,15-cyclogibberellins (9,15-cyclo-GAs) were identified by a comparison of their mass spectra and Kovats retention indices with those of authentic specimens: GA4, GA7, GA9, GA12, GA17, GA19, GA20, GA25, GA34, GA35, GA45,GA53, GA54, GA61, GA62, GA63, GA70, GA73, GA80, GA84, GA88, 3-epi-GA4, 3-epi-GA54, and 3-epi-GA63, 9,15-cyclo-GA9, lβ-hydroxy-9,15-cyclo-GA9, 2β-hydroxy-9,15-cyclo-GA9, 3α-hydroxy-9,15- cyclo-GA9, 3β-hydroxy-9,15-cyclo-GA9, and llβ-hydroxy-9,15-cyclo-GA9. The major components in the seeds at ca. 10 weeks after full-bloom were GA4, GA7, GA9, GA17, GA35, GA54, GA62, GA80, and GA84, and those in the seeds at ca. 14 weeks after full-bloom were GA17, GA25, GA45, GA62, GA63, GA80,GA84, 9,15-cyclo-GA9, and 1β-, 3β-, and 11β-hydroxy-9,15-cyclo-GA9. New GA number (GA103 _ 108) have been allocated to 9,15-cyclo-GA9, and 1β-, 2β-, 3β-, 3α-, and 11β-,hydroxy-9,15-cyclo-GA9, respectively (1β- and 3α-hydroxy-9,15-cyclo-GA9 were identified previously as fern anteridiogens, although GA numbers have not previously been assigned to these compounds). This is the first report of the co-occurrence of GA73 and 9,15-cyclo-GAs in higher plants.
采用气相色谱 - 质谱联用技术分析了苹果种子（苹果品种‘旭’）两个不同发育阶段的内源赤霉素（GAs）及与GA相关的化合物。从盛花后约10周和/或14周的种子中，通过将其质谱图和科瓦茨保留指数与标准样品进行比较，鉴定出了以下GAs和9,15 - 环赤霉素（9,15 - 环 - GAs）：GA4、GA7、GA9、GA12、GA17、GA19、GA20、GA25、GA34、GA35、GA45、GA53、GA54、GA61、GA62、GA63、GA70×GA73、GA80、GA84、GA88、3 - 表 - GA4、3 - 表 - GA54、3 - 表 - GA63、9,15 - 环 - GA9、1β - 羟基 - 9,15 - 环 - GA9、2β - 羟基 - 9,15 - 环 - GA9、3α - 羟基 - 9,15 - 环 - GA9、3β - 羟基 - 9,15 - 环 - GA9和11β - 羟基 - 9,15 - 环 - GA9。盛花后约10周种子中的主要成分是GA4、GA7、GA9、GA17、GA35、GA54、GA62、GA80和GA84，盛花后约14周种子中的主要成分是GA17、GA25、GA45、GA62、GA63、GA80、GA84、9,15 - 环 - GA9以及1β - 、3β - 和11β - 羟基 - 9,15 - 环 - GA9。已分别为9,15 - 环 - GA9以及1β - 、2β - 、3β - 、3α - 和11β - 羟基 - 9,15 - 环 - GA9分配了新的GA编号（GA103_108）（1β - 和3α - 羟基 - 9,15 - 环 - GA9先前被鉴定为蕨类植物前雄器素，尽管此前未为这些化合物指定GA编号）。这是关于GA73和9,15 - 环 - GAs在高等植物中共存的首次报道。
