Oyama N, Yamauchi T, Yamane H, Yamaguchi I, Schraudolf H, Mander L N, Murofushi N
a Department of Applied Biological Chemistry , The University of Tokyo , Tokyo , 113 , Japan.
b Biotechnology Research Center , The University of Tokyo , Tokyo , 113 , Japan.
Biosci Biotechnol Biochem. 1996 Jan;60(2):301-4. doi: 10.1271/bbb.60.301.
The following gibberellins (GAs) and antheridiogens were identified by their mass spectra and Kovats retention indices from combined gas chromatography-mass spectrometry of purified extracts of the prothallia and sporophytes of Anemia phyllitidis, a Schizaeaceous fern: a trace amount of GA9 (4-week-old prothallia); GA9, GA24, GA25, antheridic acid and 3-epi-GA63 (6-week-old prothallia); and GA4, GA9, GA15, GA19, GA20, and GA24 [young sporophytes (younger than one year old) and/or old sporophytes (between one- and two years old). Of these compounds, GA24, GA9, and GA4 were quantified by gas chromatography-selected ion monitoring, using (2)H-GAs as internal standards, and the content of antheridic acid, the principal antheridiogen, was evaluated by a radioimmunoassay which we have developed. The results indicate that endogenous levels of GAs and antheridiogens in prothallia began to increase rapidly between 4 and 6 weeks after sowing, the contents of antheridic acid and GA24, the most abundant GA in 6-week-old prothallia, being 107.4 and 37.9 ng/g fresh weight, respectively. The most abundant GA in the sporophytes was GA9, the content in young and old sporophytes being 15.3 and 7.3 ng/g fresh weight, respectively.
通过对裂叶蕨科植物贫血叶蕨原叶体和孢子体纯化提取物进行气相色谱 - 质谱联用分析，根据其质谱图和科瓦茨保留指数鉴定出了以下赤霉素（GAs）和雄器素：痕量的GA9（4周龄原叶体）；GA9、GA24、GA25、雄器酸和3 - 表 - GA63（6周龄原叶体）；以及GA4、GA9、GA15、GA19、GA20和GA24[幼龄孢子体（小于1岁）和/或老龄孢子体（1至2岁之间）]。在这些化合物中，以氘代赤霉素作为内标，通过气相色谱 - 选择离子监测对GA24、GA9和GA4进行了定量分析，并且使用我们开发的放射免疫分析法评估了主要雄器素雄器酸的含量。结果表明，原叶体中GA和雄器素的内源水平在播种后4至6周开始迅速增加，雄器酸和GA24（6周龄原叶体中含量最高的GA）的含量分别为107.4和37.9 ng/g鲜重。孢子体中含量最高的GA是GA9，幼龄和老龄孢子体中的含量分别为15.3和7.3 ng/g鲜重。
