Sugimoto Y, Uchida S, Inanaga S, Kimura Y, Hashimoto M, Isogai A
a Arid Land Research Center, Tottori University , 1390 Hamasaka, Tottori, Tottori 680 , Japan.
b Department of Agriculture , Tottori University , 4-101 Koyama-cho, Minami, Tottori, Tottori 680 , Japan.
Biosci Biotechnol Biochem. 1996 Jan;60(3):503-5. doi: 10.1271/bbb.60.503.
Cultured roots of Menispermum dauricum, a rich source of the bisbenzylisoquinoline alkaloid dauricine (1), were fed with l-[U-(14)C] tyrosine, L-[3-(13)C] tyrosine, and [2-(13)C] tvramine independently, and the incorporation of possible early precursors into 1 was studied. The results demonstrated that 1 was composed of four molecules of tyrosine, and that tyramine was specifically incorporated into the isoquinoline portions of 1. The unusual chlorine-containing alkaloid acutumine (3), into which (14)C-labeled tyrosine was also incorporated, was identified as one of the main constituents in the alkaloid fraction from the roots.
蝙蝠葛（Menispermum dauricum）的培养根富含双苄基异喹啉生物碱蝙蝠葛碱（1），分别用L-[U-(14)C]酪氨酸、L-[3-(13)C]酪氨酸和[2-(13)C]酪胺喂养，研究了可能的早期前体掺入1的情况。结果表明，1由四个酪氨酸分子组成，并且酪胺特异性掺入到1的异喹啉部分。(14)C标记的酪氨酸也掺入其中的异常含氯生物碱尖刺碱（3）被鉴定为根生物碱部分的主要成分之一。
