Bocková Markéta, Chadtová Song Xue, Gedeonová Erika, Levová Kateřina, Kalousová Marta, Zima Tomáš, Homola Jiří
Department of Optical Sensors, Institute of Photonics and Electronics of the CAS, Chaberska 57, 18251, Prague 8, Czech Republic.
Institute of Medical Biochemistry and Laboratory Diagnostics of First Faculty of Medicine, Charles University in Prague and the General University Hospital in Prague, U Nemocnice 2, 12808, Praha 2, Czech Republic.
Anal Bioanal Chem. 2016 Oct;408(26):7265-9. doi: 10.1007/s00216-016-9664-z. Epub 2016 Jun 14.
Pregnancy associated plasma protein A2 (PAPP-A2) is a metalloproteinase that plays multiple roles in fetal development and post-natal growth. Here we present a novel surface plasmon resonance (SPR) biosensor for the rapid and quantitative detection of PAPP-A2 in blood samples. This biosensor uses a single surface referencing approach and a sandwich assay with functionalized gold nanoparticles for signal enhancement. We demonstrate that this SPR biosensor enables the detection of PAPP-A2 in 30 % blood plasma at levels as low as 3.6 ng/mL. We also characterize the performance of the biosensor and evaluate its cross-reactivity to a PAPP-A analogue. Finally, we utilize this SPR biosensor for the detection of PAPP-A2 in blood serum from two groups of subjects: pregnant women and healthy non-pregnant women and men. Graphical Abstract Temporal sensor response corresponding to respective steps of the assay for detection of PAPP-A2 in buffer.
妊娠相关血浆蛋白A2（PAPP-A2）是一种金属蛋白酶，在胎儿发育和出生后生长中发挥多种作用。在此，我们展示了一种新型表面等离子体共振（SPR）生物传感器，用于快速、定量检测血液样本中的PAPP-A2。该生物传感器采用单表面参考方法和带有功能化金纳米颗粒的夹心测定法来增强信号。我们证明，这种SPR生物传感器能够在30%的血浆中检测到低至3.6 ng/mL水平的PAPP-A2。我们还对该生物传感器的性能进行了表征，并评估了其与PAPP-A类似物的交叉反应性。最后，我们利用这种SPR生物传感器检测了两组受试者血清中的PAPP-A2：孕妇以及健康的非孕妇和男性。图形摘要：对应于在缓冲液中检测PAPP-A2的测定各步骤的时间传感器响应。
