Krebs B, Schütz M, Fischer M, Sommer G, Kirchhoff H
Institut für Mikrobiologie und Tierseuchen, Tierärztliche Hochschule Hannover, Federal Republic of Germany.
Appl Environ Microbiol. 1989 Apr;55(4):852-5. doi: 10.1128/aem.55.4.852-855.1989.
The continuous cultivation of mycoplasmas in a pH-controlled metabolistat was investigated with the fermentative strain Mycoplasma mobile 163K and the nonfermentative strain Mycoplasma arthritidis ISR1. The addition of medium and the removal of culture suspension were regulated by acid production from glucose by M. mobile 163K and by ammonium production from arginine by M. arthritidis ISR1, respectively. For both strains the optimal pH for continuous growth was 7.0. The steady state could be maintained for at least 21 days. With CFU of 8.4 X 10(9) ml-1 (M. mobile 163K) and 3.2 X 10(9) ml-1 (M. arthritidis ISR1), the cell concentrations were slightly higher than those obtained in batch cultures. The dependence on the adjusted pH values was measured for several parameters, such as flow rate, CFU, glucose fermentation or production of ammonia, and gliding velocity. Since the long lag phases of batch cultures can be avoided, pH-controlled continuous cultures provide an appropriate system for the production of mycoplasma cells.
利用发酵型菌株运动支原体163K和非发酵型菌株关节炎支原体ISR1，对在pH控制的代谢恒定器中连续培养支原体进行了研究。培养基的添加和培养物悬浮液的去除分别通过运动支原体163K利用葡萄糖产酸和关节炎支原体ISR1利用精氨酸产氨来调节。对于这两种菌株，连续生长的最佳pH值均为7.0。稳定状态可维持至少21天。运动支原体163K的菌落形成单位（CFU）为8.4×10⁹ ml⁻¹，关节炎支原体ISR1的CFU为3.2×10⁹ ml⁻¹，其细胞浓度略高于分批培养获得的浓度。对几个参数进行了测定，这些参数与调节后的pH值有关，如流速、CFU、葡萄糖发酵或氨的产生以及滑动速度。由于可以避免分批培养的长延迟期，pH控制的连续培养为支原体细胞的生产提供了一个合适的系统。
