Masover G K, Hayflick L
J Bacteriol. 1974 Apr;118(1):46-52. doi: 10.1128/jb.118.1.46-52.1974.
Using dialyzing cultures of T-strain mycoplasmas, it was possible to make some observations relevant to the growth and metabolism of these organisms which would not be possible in nondialyzing cultures due to growth inhibition of the organisms by elevated pH and increased ammonium ion concentration in media containing urea. The rate of ammonia accumulation was found to be related to the initial urea concentration in the medium and could not be accounted for by any change in the multiplication rate of the organisms. More ammonia was generated than could be accounted for by the added urea alone, suggesting that an ammonia-producing activity other than urease may be present in T-strain mycoplasmas. Titers above 10(7) color change units per ml were achieved in dialysis cultures of a T-strain mycoplasma in the presence of urea, and such titers were maintained for approximately 60 h during dialysis culture in the absence of added urea.
利用T株支原体的透析培养物，有可能进行一些与这些生物体的生长和代谢相关的观察，而在非透析培养物中由于含有尿素的培养基中pH值升高和铵离子浓度增加对生物体生长的抑制作用，这些观察是不可能进行的。发现氨积累的速率与培养基中的初始尿素浓度有关，并且不能用生物体繁殖速率的任何变化来解释。产生的氨比仅由添加的尿素所能解释的要多，这表明T株支原体中可能存在除脲酶以外的产氨活性。在存在尿素的情况下，T株支原体的透析培养物中达到了每毫升高于10⁷个颜色变化单位的滴度，并且在没有添加尿素的透析培养过程中，这样的滴度维持了大约60小时。
