尿素对T株支原体生长的影响。
Influence of urea on the growth of T-strain mycoplasmas.
作者信息
Ford D K, MacDonald J
出版信息
J Bacteriol. 1967 May;93(5):1509-12. doi: 10.1128/jb.93.5.1509-1512.1967.
Abstract
T-strain mycoplasmas require urea for propagation, but urea metabolism also occurs in nonpropagating viable cultures. Ammonia results from this metabolism and alkalinizes the medium. Ammonium ions and an alkaline pH both inhibit the multiplication of T strains and reduce the viability of T strains in broth. These toxic effects of urea metabolism currently limit the growth of T strains in broth. Stock T-strain cultures are optimally maintained in continuous culture if the routine medium at pH 6.0 is supplemented with 0.05% urea and 0.002% phenol red, but an incubation temperature of 30 C is preferable to 37 C for subculture at 24-hr intervals.
摘要
T株支原体繁殖需要尿素，但在非繁殖的活培养物中也会发生尿素代谢。这种代谢会产生氨，使培养基碱化。铵离子和碱性pH值都会抑制T株的增殖，并降低其在肉汤中的活力。尿素代谢的这些毒性作用目前限制了T株在肉汤中的生长。如果在pH 6.0的常规培养基中添加0.05%尿素和0.002%酚红，T株储备培养物在连续培养中能得到最佳维持，但每隔24小时传代培养时，30℃的孵育温度比37℃更可取。
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