Almeida Jamie L, Cole Kenneth D, Plant Anne L
Biosystems and Biomaterials Division, The National Institute of Standards and Technology, Gaithersburg, Maryland, United States of America.
PLoS Biol. 2016 Jun 14;14(6):e1002476. doi: 10.1371/journal.pbio.1002476. eCollection 2016 Jun.
Different genomic technologies have been applied to cell line authentication, but only one method (short tandem repeat [STR] profiling) has been the subject of a comprehensive and definitive standard (ASN-0002). Here we discuss the power of this document and why standards such as this are so critical for establishing the consensus technical criteria and practices that can enable progress in the fields of research that use cell lines. We also examine other methods that could be used for authentication and discuss how a combination of methods could be used in a holistic fashion to assess various critical aspects of the quality of cell lines.
不同的基因组技术已应用于细胞系鉴定，但只有一种方法（短串联重复序列[STR]分析）成为全面且权威标准（ASN-0002）的主题。在此，我们讨论该文件的影响力，以及为何这样的标准对于确立能推动使用细胞系的研究领域取得进展的共识性技术标准和实践如此关键。我们还研究了可用于鉴定的其他方法，并讨论如何以整体方式结合多种方法来评估细胞系质量的各个关键方面。
