Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC), University Hospital Aachen, D-52074 Aachen, Germany.
Banco de Células do Rio de Janeiro, Rio de Janeiro 25250-020, Brazil.
Cells. 2024 Nov 10;13(22):1861. doi: 10.3390/cells13221861.
Cell culture banks play a crucial role in advancing biomedical research by providing standardized, reproducible biological materials essential for various applications, from drug development to regenerative medicine. This opinion article presents a comprehensive overview of cell culture banks, exploring their establishment, maintenance, and characterization processes. The significance of ethical considerations and regulatory frameworks governing the use of cell lines is discussed, emphasizing the importance of quality control and validation in ensuring the integrity of research outcomes. Additionally, the diverse types of cell culture banks-primary cells, immortalized cell lines, and stem cells-and their specific contributions to different fields such as cancer research, virology, and tissue engineering are examined. The impact of technological advancements on cell banking practices is also highlighted, including automation and biobanking software that enhance efficiency and data management. Furthermore, challenges faced by researchers in accessing high-quality cell lines are addressed, along with proposed strategies for improving collaboration between academic institutions and commercial entities. By unlocking the potential of cell culture banks through these discussions, this article aims to underline their indispensable role in driving innovation within biomedical research and fostering future discoveries that could lead to significant therapeutic breakthroughs.
细胞培养库在推进生物医学研究方面发挥着关键作用，为各种应用提供标准化、可重复的生物材料，从药物开发到再生医学。本文全面概述了细胞培养库，探讨了它们的建立、维护和特征描述过程。讨论了管理细胞系使用的伦理考虑因素和监管框架的重要性，强调了质量控制和验证在确保研究结果完整性方面的重要性。此外，还研究了不同类型的细胞培养库——原代细胞、永生化细胞系和干细胞，以及它们在癌症研究、病毒学和组织工程等不同领域的具体贡献。还强调了技术进步对细胞储存实践的影响，包括自动化和生物库软件，这些都提高了效率和数据管理。此外，还解决了研究人员在获取高质量细胞系方面面临的挑战，并提出了改善学术机构和商业实体之间合作的策略。通过这些讨论，本文旨在强调细胞培养库在推动生物医学研究创新和促进未来发现方面的不可或缺的作用，这些发现可能带来重大的治疗突破。
