Park Hong-Tae, Shin Min-Kyoung, Park Hyun-Eui, Cho Yong-Il, Yoo Han Sang
Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
J Vet Med Sci. 2016 Oct 1;78(9):1537-1540. doi: 10.1292/jvms.15-0271. Epub 2016 Jun 10.
Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of bovine paratuberculosis (PTB). The first step in the control of PTB is the identification and isolation of sub-clinical fecal shedders from the herd. In the current study, real-time and nested PCR targeting MAP-specific genetic elements (IS900 and ISMAP02) DNA isolated from fecal samples were used to detect MAP infection in cattle. Of the 1,562 fecal samples obtained from 37 herds, regardless of diarrhea, 35 samples tested positive in both IS900-targeted real-time and ISMAP02-targeted nested PCR. At the herd level, 12 of the 37 herds were found to be positive for MAP. Detection rates of the PCR tests were similar to those reported for ELISA-based methods. These results suggest that PCR can be used to detect sub-clinical fecal shedders of MAP.
副结核分枝杆菌（MAP）是牛副结核病（PTB）的病原体。控制PTB的第一步是从牛群中识别和分离亚临床粪便排菌者。在本研究中，针对从粪便样本中分离的MAP特异性遗传元件（IS900和ISMAP02）DNA进行实时和巢式PCR，以检测牛的MAP感染。从37个牛群中获得的1562份粪便样本，无论是否腹泻，35份样本在针对IS900的实时PCR和针对ISMAP02的巢式PCR中均检测呈阳性。在牛群水平上，37个牛群中有12个被发现MAP呈阳性。PCR检测的检出率与基于ELISA的方法报告的检出率相似。这些结果表明，PCR可用于检测MAP的亚临床粪便排菌者。
