Vandegeer Rebecca K, Powell Kevin S, Tausz Michael
Agriculture Victoria, Department of Economic Development, Jobs, Transport and Resources, AgriBio, 5 Ring Road, Bundoora, Victoria 3083, Australia; Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, 4 Water Street, Creswick, Victoria 3363, Australia.
Agriculture Victoria, Department of Economic Development, Jobs, Transport and Resources, 124 Chiltern Valley Road, Rutherglen, Victoria 3685, Australia.
J Plant Physiol. 2016 Jul 20;199:96-99. doi: 10.1016/j.jplph.2016.05.007. Epub 2016 May 20.
Plant antioxidants ascorbate and glutathione play an important role in regulating potentially harmful reactive oxygen species produced in response to virus infection. Barley yellow dwarf virus is a widespread viral pathogen that systemically infects cereal crops including wheat, barley and oats. In addition, rising atmospheric CO will alter plant growth and metabolism, including many potential but not well understood effects on plant-virus interactions. In order to better understand the wheat-BYDV interaction and any potential changes under elevated CO, the total concentration and oxidised fraction of ascorbate and glutathione was measured in leaves of a susceptible wheat cultivar (Triticum aestivum L. 'Yitpi') infected with Barley yellow dwarf virus-PAV (Padi Avenae virus) and grown under elevated CO in controlled environment chambers. Virus infection decreased total leaf ascorbate and glutathione concentrations and increased the fraction of oxidised ascorbate (dehydroascorbate). Elevated CO decreased the fraction of oxidised ascorbate. In this work, we demonstrate that systemic infection by a phloem-restricted virus weakens the antioxidant pools of ascorbate and glutathione. In addition, elevated CO may decrease oxidative stress, for example, from virus infection, but there was no direct evidence for an interactive effect between treatments.
植物抗氧化剂抗坏血酸和谷胱甘肽在调节因病毒感染而产生的潜在有害活性氧物种方面发挥着重要作用。大麦黄矮病毒是一种广泛传播的病毒病原体，可系统性感染包括小麦、大麦和燕麦在内的谷类作物。此外，大气中二氧化碳浓度升高会改变植物的生长和代谢，包括对植物与病毒相互作用产生许多潜在但尚未完全了解的影响。为了更好地理解小麦与大麦黄矮病毒的相互作用以及在二氧化碳浓度升高情况下的任何潜在变化，我们对在可控环境舱中生长并感染了大麦黄矮病毒 - PAV（燕麦缢管病毒）的易感小麦品种（普通小麦‘Yitpi’）叶片中的抗坏血酸和谷胱甘肽的总浓度及氧化部分进行了测量。病毒感染降低了叶片中抗坏血酸和谷胱甘肽的总浓度，并增加了氧化型抗坏血酸（脱氢抗坏血酸）的比例。二氧化碳浓度升高降低了氧化型抗坏血酸的比例。在这项研究中，我们证明韧皮部限制型病毒的系统性感染会削弱抗坏血酸和谷胱甘肽的抗氧化库。此外，二氧化碳浓度升高可能会降低例如由病毒感染引起的氧化应激，但没有直接证据表明处理之间存在交互作用。
