Santilli I, Prelle A, Geremia L, Scarlato G, Meola G
Istituto di Clinica Neurologica, Centro Dino Ferrari, Università degli Studi, Milano, Italy.
Basic Appl Histochem. 1989;33(1):49-52.
We have applied a new fluorescent probe, Nile red, on normal and pathological human muscle derived cultures and compared the results with corresponding human muscle sections. In normal human muscle cultures, Nile red strain has proved useful for visualization of both intracellular lipids and membrane network. Similar patterns have been observed in muscle cultures derived from lipid storage and mitochondrial myopathies. Moreover, abnormalities in pathological muscle cultures could be revealed by establishing more advanced culture systems.
我们已将一种新型荧光探针尼罗红应用于正常和病理状态下的人肌肉衍生培养物，并将结果与相应的人肌肉切片进行了比较。在正常人肌肉培养物中，尼罗红染色已被证明可用于可视化细胞内脂质和膜网络。在脂质贮积性和线粒体肌病衍生的肌肉培养物中也观察到了类似的模式。此外，通过建立更先进的培养系统，可以揭示病理肌肉培养物中的异常情况。
