Bonilla E, Prelle A
J Histochem Cytochem. 1987 May;35(5):619-21. doi: 10.1177/35.5.3559182.
Using frozen sections from human muscle biopsies, we assessed the value of Nile blue and Nile red, two fluorescent probes, as stains for lipid droplets in normal and pathological skeletal muscle fibers. In normal muscle, lipid storage disorders, and mitochondrial myopathies, Nile blue stained the lipid droplets as yellow-gold fluorescent structures. The lipid droplets were also seen as yellow-gold fluorescent structures in Nile red-stained sections, but the outstanding feature in these preparations was the staining of the membrane network of the muscle fibers and membrane proliferations in pathological muscle as red-orange fluorescent structures. These results suggest that both Nile blue and Nile red stains are useful for visualization of lipid droplets and membrane proliferations in pathological muscle biopsies.
我们使用人类肌肉活检的冰冻切片，评估了两种荧光探针尼罗蓝和尼罗红作为正常和病理性骨骼肌纤维中脂滴染色剂的价值。在正常肌肉、脂质贮积病和线粒体肌病中，尼罗蓝将脂滴染成金黄荧光结构。在尼罗红染色切片中，脂滴也呈现为金黄荧光结构，但这些标本中的突出特征是肌肉纤维的膜网络和病理性肌肉中的膜增生被染成红橙色荧光结构。这些结果表明，尼罗蓝和尼罗红染色剂都有助于在病理性肌肉活检中观察脂滴和膜增生。
