Tischler U, Rückert D, Schubert R, Jaroni H W, Schmidt K H
Department of Surgery, Chirurgische Klinik und Poliklinik, University of Tübingen, West Germany.
Biochem Biophys Res Commun. 1989 May 15;160(3):1219-26. doi: 10.1016/s0006-291x(89)80133-0.
Interaction of large unilamellar phosphatidylcholine vesicles (LUV, 75nm) and plasma high density lipoproteins (HDL) resulted in a non-leaky vesiculation of LUV. This vesiculation was detected by a HPLC-system consisting of a combination of three TSK-gel columns (6000PW, 5000PW, 3000SW). With increasing incubation time liposomal [14C]PC, entrapped [3H]inulin, and apoprotein of HDL origin decreased. The decrease was accompanied by a formation of new particles, consisting of liposomal PC and apoprotein. These particles also enclosed [3H]inulin, reflecting a hydrophilic inner space. The formation of the particles reached a maximum after one day of incubation. Retention time was 21 minutes for LUV, 28 minutes for the new particles, and 36 minutes for HDL. In vesicles with membranes consisting of phosphatidylcholine and 30% cholesterol no interactions were observed.
大单层磷脂酰胆碱囊泡（LUV，75纳米）与血浆高密度脂蛋白（HDL）的相互作用导致LUV发生非渗漏性囊泡化。这种囊泡化通过由三根TSK凝胶柱（6000PW、5000PW、3000SW）组合而成的高效液相色谱系统检测到。随着孵育时间的增加，脂质体[14C]PC、包封的[3H]菊粉以及HDL来源的载脂蛋白减少。这种减少伴随着由脂质体PC和载脂蛋白组成的新颗粒的形成。这些颗粒也包封了[3H]菊粉，反映出一个亲水性的内部空间。颗粒的形成在孵育一天后达到最大值。LUV的保留时间为21分钟，新颗粒为28分钟，HDL为36分钟。在由磷脂酰胆碱和30%胆固醇组成膜的囊泡中未观察到相互作用。
