Bienvenüe A, Vidal M, Sainte-Marie J, Philippot J
Biochim Biophys Acta. 1985 Jul 31;835(3):557-66. doi: 10.1016/0005-2760(85)90125-0.
The kinetics of spin-labeled phosphatidylcholine transfer between vesicles and HDL particles exhibited a two-phase process, as seen by ESR spectroscopy. The results were analyzed by considering several possible steps in the overall transfer, whose aspects were also studied: (i) micellar complex formation after HDL apolipoprotein-vesicle mixture, (ii) the rate of PC transfer from the micellar complex to HDL, (iii) the rate of the reverse reaction between overloaded HDL particles and other particles such as HDLs, LDLs, and lipid vesicles. The results agree most convincingly with a mechanism in which the diffusion of phospholipids into the HDL-endogenous lipids is the limiting step, occurring as a two-step process. In addition, we observed a negative charge effect on the lipid transfer rates and yields.
通过电子自旋共振光谱法观察到，自旋标记的磷脂酰胆碱在囊泡和高密度脂蛋白（HDL）颗粒之间的转移动力学呈现出一个两相过程。通过考虑整体转移中的几个可能步骤对结果进行了分析，这些步骤的各个方面也进行了研究：（i）HDL载脂蛋白 - 囊泡混合物后胶束复合物的形成，（ii）磷脂酰胆碱从胶束复合物转移到HDL的速率，（iii）过载的HDL颗粒与其他颗粒（如HDL、低密度脂蛋白（LDL）和脂质囊泡）之间的逆反应速率。结果最令人信服地符合一种机制，即磷脂向HDL内源性脂质的扩散是限速步骤，该过程以两步进行。此外，我们观察到了电荷对脂质转移速率和产率的负效应。
